SerpinF1 / PEDF Recombinant Protein | PEDF recombinant protein

Recombinant Human SerpinF1 / PEDF Protein (His tag)

Cat. Num. AAA2545912

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: SerpinF1 / PEDF; Recombinant Human SerpinF1 / PEDF Protein (His tag); EPC-1; PEDF; PIG35; SERPINF1; PEDF recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 418

• Application Notes: The secreted recombinant human SERPINF1 consists of 410 amino acids with the predicted molecular mass of 45.8 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gln 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PEDF recombinant protein

Background: Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.

Description: A DNA sequence encoding the human SERPINF1 (NP_002606.3) (Met 1-Pro 418) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NM_002615.6
• UniProt Accession #: P36955; Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092
• Molecular Weight: 46,312 Da
• NCBI Official Full Name: pigment epithelium-derived factor isoform 1
• NCBI Official Synonym Full Names: serpin family F member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor
• UniProt Protein Name: Pigment epithelium-derived factor
• UniProt Gene Name: SERPINF1
• UniProt Synonym Gene Names: PEDF; PEDF

NCBI Description

This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016]

Uniprot Description

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: protein binding; serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; multicellular organismal development; negative regulation of angiogenesis; positive regulation of neurogenesis

Disease: Osteogenesis Imperfecta, Type Vi