Human PEDF/SerpinF1 PicoKine ELISA Kit | SERPINF1 elisa kit

Human PEDF/SerpinF1 PicoKine ELISA Kit

Cat. Num. AAA177603

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Reactivity: Human
• Synonyms: PEDF/SerpinF1 PicoKine; Human PEDF/SerpinF1 PicoKine ELISA Kit; Pigment epithelium-derived factor; Alpha 2 antiplasmin; Cell proliferation-inducing gene 35 protein; EPC 1; EPC-1; EPC1; OI12; OI6; PEDF; PEDF_HUMAN; PIG 35; PIG35; Pigment epithelium derived factor; Proliferation inducing protein 35; serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived factor); member 1; SERPIN F1; Serpin peptidase inhibitor clade F member 1; serpin peptidase inhibitor; SERPINF 1; SERPINF1 ; SERPINF1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human PEDF
• Sequence Length: 418

• Assay Range: 0.78ng/ml-50ng/ml
• Sensitivity: <20pg/ml
• Sample: For quantitative detection of human PEDF in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, M1-P418
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml human PEDF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human PEDF standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(ng/ml)|5.62|10.1|25.5|6.23|12.1|26.4; Standard deviation|0.259|0.444|1.07|0.467|0.835|1.95; CV(%)|4.6|4.4|4.2|7.5|6.9|7.4
• Sample Data: Concentration(ng/ml)|0|0.78|1.56|3.12|6.25|12.5|25|50; O.D.|0.051|0.101|0.134|0.243|0.439|0.829|1.482|2.472
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human PEDF/SerpinF1 PicoKine ELISA Kit standard curve )

Related Product Information for SERPINF1 elisa kit

Principle of the assay: Human PEDF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PEDF has been precoated onto 96-well plates. Standards(NSO, M1-P418) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PEDF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PEDF amount of sample captured in plate.

Background: Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NM_002615.5
• UniProt Accession #: P36955; Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092
• Molecular Weight: 46,312 Da
• NCBI Official Full Name: pigment epithelium-derived factor
• NCBI Official Synonym Full Names: serpin family F member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor
• UniProt Protein Name: Pigment epithelium-derived factor
• Protein Family: Pigment epithelium-derived factor
• UniProt Gene Name: SERPINF1
• UniProt Synonym Gene Names: PEDF; PEDF
• UniProt Entry Name: PEDF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

Uniprot Description

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; basement membrane; extracellular matrix; extracellular region; extracellular space; melanosome; perinuclear region of cytoplasm

Molecular Function: protein binding; serine-type endopeptidase inhibitor activity

Biological Process: aging; cell proliferation; kidney development; multicellular organismal development; negative regulation of angiogenesis; negative regulation of inflammatory response; ovulation cycle; positive regulation of neurogenesis; response to acidity; response to arsenic; retina development in camera-type eye; short-term memory

Disease: Osteogenesis Imperfecta, Type Vi