Typical Testing Data/Standard Curve (for reference only)
Mouse Serine protease inhibitor A3N ELISA Kit | Serpina3n elisa kit
Mouse Serine protease inhibitor A3N ELISA Kit
Reactivity
Mouse
Synonyms
Serine protease inhibitor A3N; Mouse Serine protease inhibitor A3N ELISA Kit; Serpina3n elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouse Serpin A3N. No significant cross-reactivity or interference was observed.
Sequence Length
408
Samples
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Serpina3n elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
46,718 Da
NCBI Official Full Name
Serine protease inhibitor A3N
UniProt Protein Name
Serine protease inhibitor A3N
Protein Family
UniProt Gene Name
Serpina3n
UniProt Synonym Gene Names
Spi2; Serpin A3N
UniProt Entry Name
SPA3N_MOUSE
Uniprot Description
SERPINA3: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: protease inhibitor activity; serine-type endopeptidase inhibitor activity
Biological Process: acute-phase response; negative regulation of peptidase activity; response to cytokine stimulus; response to peptide hormone stimulus
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Product Notes
The Mouse Serpina3n serpina3n (Catalog #AAA288366) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288366 ELISA Kit recognizes Mouse Serpina3n. It is sometimes possible for the material contained within the vial of "Serine protease inhibitor A3N, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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