Typical Testing Data/Standard Curve (for reference only)
Mouse Plasminogen activator inhibitor 1 ELISA Kit | Serpine1 elisa kit
Mouse Plasminogen activator inhibitor 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Cellular Component: extracellular matrix; extracellular region; extracellular space
Molecular Function: protease binding; protease inhibitor activity; protein binding; receptor binding; serine-type endopeptidase inhibitor activity
Biological Process: defense response to Gram-negative bacterium; female gonad development; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of fibrinolysis; negative regulation of peptidase activity; negative regulation of smooth muscle cell migration; placenta development; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of coagulation; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of keratinocyte migration; positive regulation of receptor-mediated endocytosis; regulation of angiogenesis; regulation of cell proliferation; regulation of receptor activity; wound healing
Research Articles on Serpine1
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Product Notes
The Mouse Serpine1 serpine1 (Catalog #AAA2882106) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882106 ELISA Kit recognizes Mouse Serpine1. It is sometimes possible for the material contained within the vial of "Plasminogen activator inhibitor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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