Human Prostasin ELISA Kit | PRSS8 elisa kit

Human Prostasin ELISA Kit

Cat. Num. AAA2882250

• Gene Names: PRSS8; CAP1; PROSTASIN
• Reactivity: Human
• Synonyms: Prostasin; Human Prostasin ELISA Kit; Serine protease 8; PRSS8; 3.4.21.-; CAP1; PRSS8 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Prostasin. No significant cross-reactivity or interference was observed.
• Sequence Length: 343

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRSS8 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506153
• NCBI GeneID: 5652
• NCBI Accession #: NP_002764.1
• NCBI GenBank Nucleotide #: NM_002773.4
• UniProt Accession #: Q16651; Q9UCA3; B4DWP2
• Molecular Weight: 30,519 Da
• NCBI Official Full Name: prostasin preproprotein
• NCBI Official Synonym Full Names: protease, serine 8
• NCBI Official Symbol: PRSS8
• NCBI Official Synonym Symbols: CAP1; PROSTASIN
• NCBI Protein Information: prostasin
• UniProt Protein Name: Prostasin
• Protein Family: Prostasin
• UniProt Gene Name: PRSS8
• UniProt Synonym Gene Names: CAP1
• UniProt Entry Name: PRSS8_HUMAN

NCBI Description

This gene encodes a member of the peptidase S1 or chymotrypsin family of serine proteases. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate via a disulfide bond to form the heterodimeric enzyme. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. This protease exhibits trypsin-like substrate specificity, cleaving protein substrates at the carboxyl terminus of lysine or arginine residues. The encoded protease partially mediates proteolytic activation of the epithelial sodium channel, a regulator of sodium balance, and may also play a role in epithelial barrier formation. [provided by RefSeq, Feb 2016]

Uniprot Description

PRSS8: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular region; extracellular space; integral to membrane; plasma membrane

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis