Mouse Serine protease inhibitor A3G ELISA Kit | Serpina3g elisa kit

Mouse Serine protease inhibitor A3G ELISA Kit

Cat. Num. AAA761527

• Gene Names: Serpina3g; 2A2; spi2; Spi2A; Spi2-1; AI119734; Spi2/eb.1
• Reactivity: Mouse
• Synonyms: Serine protease inhibitor A3G; Mouse Serine protease inhibitor A3G ELISA Kit; Serpina3g/Spi2A/Serpin A3G/Serine protease inhibitor 2A/Serpin 2A; Serpina3g elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of EPX. No significant cross-reactivity or interference between EPX and analogues was observed.
• Sequence Length: 440

• Samples: Cell, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Serpina3g elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 86476056
• NCBI GeneID: 20715
• NCBI Accession #: NP_033277.1
• NCBI GenBank Nucleotide #: NM_009251.1
• Molecular Weight: 49,021 Da
• NCBI Official Full Name: serine protease inhibitor A3G
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade A, member 3G
• NCBI Official Symbol: Serpina3g
• NCBI Official Synonym Symbols: 2A2; spi2; Spi2A; Spi2-1; AI119734; Spi2/eb.1
• NCBI Protein Information: serine protease inhibitor A3G
• UniProt Protein Name: Serine protease inhibitor A3G
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: Serpina3g
• UniProt Synonym Gene Names: Spi2A; Serpin A3G; Serpin 2A
• UniProt Entry Name: SPA3G_MOUSE

Uniprot Description

Serpina3g: Serine and cysteine protease inhibitor. Can inhibit lysosomal papain-like proteases including the cathepsins B, G, H, K, L and V. Ineffective against elastase, granzyme A, granzyme B, or caspases 3, 8 or 9. Inhibition of cytoplasmic cathepsin B following release from the lysosome may protect cells from apoptosis. This may facilitate the survival of progenitor T-cells and the subsequent development of long term memory CD8 T-cells. Belongs to the serpin family.

Cellular Component: cytoplasm; extracellular space; nucleus

Molecular Function: cysteine protease inhibitor activity; protease inhibitor activity; serine-type endopeptidase inhibitor activity

Biological Process: adaptive immune response; apoptosis; immune system process; negative regulation of peptidase activity; response to cytokine stimulus; response to peptide hormone stimulus