Rabbit anti-Human Cytochrome p450 2C9 Polyclonal Antibody | anti-CYP2C9 antibody

Cytochrome p450 2C9, NT (CYP2C9, (R)(S)-limonene 6-monooxygenase, (S)-limonene 6-monooxygenase, Cytochrome P450 PB-1, Cytochrome P450 MP-4, Cytochrome P450 MP-8, S-mephenytoin 4-hydroxylase) (PE)

Cat. Num. AAA6471478

• Gene Names: CYP2C9; CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Cytochrome p450 2C9; Polyclonal Antibody; NT (CYP2C9; (R)(S)-limonene 6-monooxygenase; (S)-limonene 6-monooxygenase; Cytochrome P450 PB-1; Cytochrome P450 MP-4; Cytochrome P450 MP-8; S-mephenytoin 4-hydroxylase) (PE); anti-CYP2C9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP2C9.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CYP2C9 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human CYP2C9 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CYP2C9 Antibody (N-term) in Jurkat cell line lysates (35ug/lane). CYP2C9(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CYP2C9 Antibody (N-term) (AP7874a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP2C9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-CYP2C9 antibody

Antibodies; Enzymes; Cytochrome

References

1. Matimba, A., Hum. Genomics 3(2), 169-190 (2009). 2. Nelson, D.R., Pharmacogenetics 14(1), 1-18 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 13699818
• NCBI GeneID: 1559
• NCBI Accession #: NP_000762
• NCBI GenBank Nucleotide #: NM_000771.3
• UniProt Accession #: P11712; P11713; Q16756; Q16872; Q5VX92; Q6IRV8; Q8WW80
• Molecular Weight: 18,005 Da
• NCBI Official Full Name: cytochrome P450 2C9
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily C, polypeptide 9
• NCBI Official Symbol: CYP2C9
• NCBI Official Synonym Symbols: CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• NCBI Protein Information: cytochrome P450 2C9
• UniProt Protein Name: Cytochrome P450 2C9
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2C9
• UniProt Synonym Gene Names: CYP2C10
• UniProt Entry Name: CP2C9_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]