SEPW1 recombinant protein

Recombinant human SEPW1 protein

Cat. Num. AAA201346

• Gene Names: SELENOW; selW; SEPW1
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: SEPW1; Recombinant human SEPW1 protein; Selenoprotein W; selW; SEPW1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.25mg/ml (determined by BRADFORD assay) (varies by lot)
• Sequence Positions: 1-87aa
• Sequence: MALAVRV VYCGACGYKS KYLQLKKKLE DEFPGRLDIC GEGTPQATGF FEVMVAGKLI HSKKKGDGYV DTESKFLKLV AAIKAALAQG

• Applicable Applications for SEPW1 recombinant protein: SDS-PAGE
• Species: Human
• Tag: His-Tag
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for SEPW1 recombinant protein

SEPW1 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3 uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. This protein shows highest expression in skeletal muscle and heart, and may be involved in oxidation-reduction reactions. A retroprocessed pseudogene, SEPW1P, has been identified and mapped to chromosome 1p35-34. Recombinant human SEPW1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SEPW1 recombinant protein

Other; Proteins

References

Hawkes,W.C., et al. (2012) J. Biol. Chem. 287 (33), 27371-27379; Hawkes,W.C., et al. (2012) J. Cell. Biochem. 113 (1), 61-69

NCBI and Uniprot Product Information

• NCBI GI #: 4506887
• NCBI GeneID: 6415
• NCBI Accession #: NP_003000
• NCBI GenBank Nucleotide #: NM_003009.3
• UniProt Accession #: P63302; O15532; O19096; Q86TI9; Q96KM5; A8MRR7
• Molecular Weight: 11.8kDa (110aa) confirmed by MALDI-TOF
• NCBI Official Full Name: selenoprotein W
• NCBI Official Synonym Full Names: selenoprotein W
• NCBI Official Symbol: SELENOW
• NCBI Official Synonym Symbols: selW; SEPW1
• NCBI Protein Information: selenoprotein W
• UniProt Protein Name: Selenoprotein W
• Protein Family: Selenoprotein
• UniProt Gene Name: SEPW1
• UniProt Synonym Gene Names: SELW; SelW
• UniProt Entry Name: SELW_HUMAN

NCBI Description

This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle, heart and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A retroprocessed pseudogene of this locus has been identified on chromosome 1. [provided by RefSeq, Aug 2017]

Uniprot Description

SEPW1: Plays a role as a glutathione (GSH)-dependent antioxidant. May be involved in a redox-related process. May play a role in the myopathies of selenium deficiency. Belongs to the SelWTH family. SELW subfamily.

Chromosomal Location of Human Ortholog: 19q13.3