SENP8 recombinant protein
Recombinant Human SENP8 Protein
Gene Names
SENP8; DEN1; NEDP1; PRSC2
Reactivity
Human
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Synonyms
SENP8; Recombinant Human SENP8 Protein; DEN1; NEDP1; PRSC2; Sentrin-specific protease 8; Deneddylase-1; NEDD8-specific protease 1; Protease; cysteine 2; Sentrin/SUMO-specific protease SENP8; SENP8 recombinant protein
Host
E Coli
Reactivity
Human
Purity/Purification
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Sequence
MGSSHHHHHH SSGLVPRGSH MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLIATLA KK
Sequence Length
212
Applicable Applications for SENP8 recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for SENP8 recombinant protein
Recombinant Human SENP8 Protein is produced by our E Coli expression system and the target gene encoding Met1-Lys212 is expressed with a 6His tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,107 Da
NCBI Official Full Name
sentrin-specific protease 8
NCBI Official Synonym Full Names
SUMO/sentrin peptidase family member, NEDD8 specific
NCBI Official Symbol
SENP8
NCBI Official Synonym Symbols
DEN1; NEDP1; PRSC2
NCBI Protein Information
sentrin-specific protease 8
UniProt Protein Name
Sentrin-specific protease 8
Protein Family
UniProt Gene Name
SENP8
UniProt Synonym Gene Names
DEN1; NEDP1; PRSC2
UniProt Entry Name
SENP8_HUMAN
NCBI Description
This gene encodes a cysteine protease that is a member of the sentrin-specific protease family. The encoded protein is involved in processing and deconjugation of the ubiquitin-like protein termed, neural precursor cell expressed developmentally downregulated 8. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]
Uniprot Description
SENP8: Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as cullins or p53. Belongs to the peptidase C48 family.
Protein type: EC 3.4.22.68; Protease
Chromosomal Location of Human Ortholog: 15q23
Research Articles on SENP8
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Product Notes
The SENP8 senp8 (Catalog #AAA8249757) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human SENP8 Protein reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SENP8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the SENP8 senp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MDPVVLSYMD SLLRQSDVSL LDPPSWLNDH IIGFAFEYFA NSQFHDCSDH VSFISPEVTQ FIKCTSNPAE IAMFLEPLDL PNKRVVFLAI NDNSNQAAGG THWSLLVYLQ DKNSFFHYDS HSRSNSVHAK QVAEKLEAFL GRKGDKLAFV EEKAPAQQNS YDCGMYVICN TEALCQNFFR QQTESLLQLL TPAYITKKRG EWKDLIATLA KK. It is sometimes possible for the material contained within the vial of "SENP8, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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