Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SENP6 recombinant protein
SENP6, Active
Synonyms
SENP6; Active; SUMO-1-specific protease 6; SUSP1; SSP1; KIAA0797; SENP6 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Tag
HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for SENP6 recombinant protein
Recombinant human SENP6 (637-1112) was expressed in E Coli cells using an N-terminal His tag. The SENP6 gene accession number is NM_015571.
SENP6 is a mammalian SUMO protease that preferentially disassembles conjugated SUMO2/3 chains. It is found in the nucleoplasm and possess a conserved sequence insertion within the catalytic domain which is absent in the catalytic domains of the other SENP family members (1). SENP6 has been shown to be essential for cell proliferation and cell cycle progression with a prominent role during mitosis (2). SENP6 depleted cells exhibit defects in the spindle assembly and metaphase chromosome congression resulting in mitotic problems and cell death. Thus, SENP6 inhibitors have clinical potential against highly proliferative cancer cells (3).
SENP6 is a mammalian SUMO protease that preferentially disassembles conjugated SUMO2/3 chains. It is found in the nucleoplasm and possess a conserved sequence insertion within the catalytic domain which is absent in the catalytic domains of the other SENP family members (1). SENP6 has been shown to be essential for cell proliferation and cell cycle progression with a prominent role during mitosis (2). SENP6 depleted cells exhibit defects in the spindle assembly and metaphase chromosome congression resulting in mitotic problems and cell death. Thus, SENP6 inhibitors have clinical potential against highly proliferative cancer cells (3).
Product Categories/Family for SENP6 recombinant protein
References
1. Choi SJ, et al: (2001) Negative modulation of RXRalpha transcriptional activity by small ubiquitin-related modifier (SUMO) modification and its reversal by SUMO-specific protease SUSP1. J. Biol. Chem. 281, 30669-30677.
2. Mukhopadhyay D, et al: (2010) The SUMO protease SENP6 is essential for inner kinetochore assembly. J Cell Biol. 2010;188(5):681?692.
3. Liebelt F, et al: (2019) The poly-SUMO2/3 protease SENP6 enables assembly of the constitutive centromere-associated network by group deSUMOylation. Nat Commun. 10, 3987.
2. Mukhopadhyay D, et al: (2010) The SUMO protease SENP6 is essential for inner kinetochore assembly. J Cell Biol. 2010;188(5):681?692.
3. Liebelt F, et al: (2019) The poly-SUMO2/3 protease SENP6 enables assembly of the constitutive centromere-associated network by group deSUMOylation. Nat Commun. 10, 3987.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64kDa
Protein Family
Similar Products
Product Notes
The SENP6 (Catalog #AAA517600) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SENP6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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