SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
OTUB1 recombinant protein
OTUB1, Protein
Applications
Western Blot
Synonyms
OTUB1; Protein; OTB1; OTU1; HSPC263; OTU deubiquitinase; ubiquitin aldehyde binding 1; OTUB1 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for OTUB1 recombinant protein
Western Blot (WB)
Tag
HIS
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for OTUB1 recombinant protein
Recombinant full-length human OTUB1 was expressed in E Coli cells using an N-terminal His tag. The OTUB1 gene accession number is NM_017670.
OTUB1 (OTU domain-containing ubiquitin aldehyde-binding protein 1), as a member of the ovarian tumor (OUT) superfamily of predicted cysteine proteases (1). It specifically cleaves ubiquitin from branched lysine48-linked poly-ubiquitin chains (1). OTUB1 inhibits the synthesis of the K63-linked polyubiquitin chains through binding to the UBC13 (2). Overexpression of OTUB1 is associated to poor outcome in some breast cancer patients with chemotherapy (3).
OTUB1 (OTU domain-containing ubiquitin aldehyde-binding protein 1), as a member of the ovarian tumor (OUT) superfamily of predicted cysteine proteases (1). It specifically cleaves ubiquitin from branched lysine48-linked poly-ubiquitin chains (1). OTUB1 inhibits the synthesis of the K63-linked polyubiquitin chains through binding to the UBC13 (2). Overexpression of OTUB1 is associated to poor outcome in some breast cancer patients with chemotherapy (3).
Product Categories/Family for OTUB1 recombinant protein
References
1. Edelmann MJ, et al. Structural basis and specificity of human otubain 1-mediated deubiquitination. Biochem J. 2009 Mar 1;418(2):379-90.
2. Wiener R, et al. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22.
3. Karunarathna U, et al. OTUB1 inhibits the ubiquitination and degradation of FOXM1 in breast cancer and epirubicin resistance. Oncogene. 2016 Mar 17;35(11):1433-44.
2. Wiener R, et al. The mechanism of OTUB1-mediated inhibition of ubiquitination. Nature. 2012 Feb 22;483(7391):618-22.
3. Karunarathna U, et al. OTUB1 inhibits the ubiquitination and degradation of FOXM1 in breast cancer and epirubicin resistance. Oncogene. 2016 Mar 17;35(11):1433-44.
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35kDa
Protein Family
Similar Products
Product Notes
The OTUB1 (Catalog #AAA517349) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's OTUB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the OTUB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTUB1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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