Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SEMA4D(CD100) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Mouse anti-Human Semaphorin 4D Monoclonal Antibody | anti-SEMA4D antibody
Anti-Semaphorin 4D Antibody, Mouse Monoclonal
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
Semaphorin 4D; Monoclonal Antibody; Anti-Semaphorin 4D Antibody; Mouse Monoclonal; Semaphorin 4D/SEMA4D/CD100 Antibody; Mouse MAb; semaphorin 4D; Anti-C9orf164 Antibody; Anti-CD100 Antibody; Anti-coll-4 Antibody; Anti-COLL4 Antibody; Anti-M-sema-G Antibody; Anti-SEMAJ Antibody; anti-SEMA4D antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
05
Specificity
Human Semaphorin 4D/SEMA4D/CD100
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SEMA4D antibody
Flow Cytometry (FC/FACS/FCM)
Application Notes
FC/FACS/FCM: 1:25-1:100
Immunogen
Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Semaphorin 4D/SEMA4D/CD100 (rh Semaphorin 4D/SEMA4D/CD100). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SEMA4D(CD100) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human SEMA4D(CD100) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Related Product Information for anti-SEMA4D antibody
Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The SEMA4D (Catalog #AAA8105838) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Semaphorin 4D Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 4D can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the SEMA4D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Semaphorin 4D, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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