Human Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) ELISA Kit | TERF2IP elisa kit

Human Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) ELISA Kit

Cat. Num. AAA453737

• Gene Names: TERF2IP; RAP1; DRIP5
• Reactivity: Human
• Synonyms: Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP); Human Telomeric Repeat Binding Factor 2 Interacting Protein (TERF2IP) ELISA Kit; RAP1; DRIP5; Dopamine receptor-interacting protein 5; Repressor/activator protein 1 homolog; TERF2IP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TERF2IP. No significant cross-reactivity or interference between TERF2IP and analogues was observed.
• Sequence Length: 399

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.66ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TERF2IP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TERF2IP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TERF2IP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TERF2IP in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TERF2IP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TERF2IP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TERF2IP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TERF2IP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 52627149
• NCBI GeneID: 54386
• NCBI Accession #: NP_061848.2
• NCBI GenBank Nucleotide #: NM_018975.3
• UniProt Accession #: Q9NYB0; Q4W4Y2; Q8WYZ3; Q9NWR2; B4DQN4
• Molecular Weight: 44,260 Da
• NCBI Official Full Name: telomeric repeat-binding factor 2-interacting protein 1
• NCBI Official Synonym Full Names: TERF2 interacting protein
• NCBI Official Symbol: TERF2IP
• NCBI Official Synonym Symbols: RAP1; DRIP5
• NCBI Protein Information: telomeric repeat-binding factor 2-interacting protein 1
• UniProt Protein Name: Telomeric repeat-binding factor 2-interacting protein 1
• Protein Family: Telomeric repeat-binding factor 2-interacting protein
• UniProt Gene Name: TERF2IP
• UniProt Synonym Gene Names: DRIP5; RAP1; TERF2-interacting telomeric protein 1; TRF2-interacting telomeric protein 1; RAP1 homolog; hRap1

NCBI Description

The gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22. [provided by RefSeq, Apr 2010]

Uniprot Description

Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.