Semaphorin-4A/SEMA4A Recombinant Protein | SEMA4A recombinant protein

Recombinant Mouse Semaphorin-4A/SEMA4A Protein (C-6His)

Cat. Num. AAA2553607

• Gene Names: Sema4a; SemB; Semab; AI132332
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: Semaphorin-4A/SEMA4A; Recombinant Mouse Semaphorin-4A/SEMA4A Protein (C-6His); Semaphorin-4A; Semaphorin-B; Sema B; Sema4a; Semab; SemB; SEMA4A recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence Positions: Thr33-His682
• Sequence Length: 760

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fusion Tag: C-6His
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water(50ul) be added to the vial to prepare astock solution of 0.2mg/mL.; Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at < -20 to 80°C.; Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.; This product is provided as lyophilized powder which is shipped in blue ice.

SDS-PAGE

Related Product Information for SEMA4A recombinant protein

Semaphorin-4A (SEMA4A) belongs to the semaphorin family which contains a Ig-like C2-type domain, a PSI domain and a Sema domain. SEMA4A is expressed from day 10 in the embryo, and low levels are found between days 10-12. SEMA4A is a cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. It plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. SEMA4A promotes phosphorylation of TIMD2, inhibits angiogenesis, and promotes axon growth cone collapse, Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

NCBI and Uniprot Product Information

• NCBI GI #: 254553354
• NCBI GeneID: 20351
• NCBI Accession #: NP_001156961.1
• NCBI GenBank Nucleotide #: NM_001163489.1
• UniProt Accession #: Q62178; D3Z5L2
• Molecular Weight: Molecular Mass: 72.7 kDa; Actual Protein Molecular Mass: 70-90 kDa
• NCBI Official Full Name: semaphorin-4A
• NCBI Official Synonym Full Names: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
• NCBI Official Symbol: Sema4a
• NCBI Official Synonym Symbols: SemB; Semab; AI132332
• NCBI Protein Information: semaphorin-4A
• UniProt Protein Name: Semaphorin-4A
• Protein Family: Semaphorin
• UniProt Gene Name: Sema4a
• UniProt Synonym Gene Names: Semab; SemB; Sema B

Uniprot Description

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.