Rabbit anti-Human RASSF3 Polyclonal Antibody | anti-RASSF3 antibody

RASSF3, NT (RASSF3, Ras association domain-containing protein 3) (FITC)

Cat. Num. AAA6340512

• Gene Names: RASSF3; RASSF5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RASSF3; Polyclonal Antibody; NT (RASSF3; Ras association domain-containing protein 3) (FITC); Ras association domain-containing protein 3; anti-RASSF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RASSF3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RASSF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-36 amino acids from the N-terminal region of human RASSF3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RASSF3 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RASSF3 antibody detected the RASSF3 protein (arrow).)

Related Product Information for anti-RASSF3 antibody

The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM].

Product Categories/Family for anti-RASSF3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Jacquemart, I.C., et al. Int. J. Oncol. 34(2):517-528(2009). Fujita, H., et al. J. Biol. Chem. 280(6):5022-5031(2005). Tommasi, S., et al. Oncogene 21(17):2713-2720(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 30039698
• NCBI GeneID: 283349
• NCBI Accession #: NP_835463.1
• NCBI GenBank Nucleotide #: NM_178169.3
• UniProt Accession #: Q86WH2; Q86WH1
• Molecular Weight: 10,265 Da
• NCBI Official Full Name: ras association domain-containing protein 3
• NCBI Official Synonym Full Names: Ras association (RalGDS/AF-6) domain family member 3
• NCBI Official Symbol: RASSF3
• NCBI Official Synonym Symbols: RASSF5
• NCBI Protein Information: ras association domain-containing protein 3; Ras association (RalGDS/AF-6) domain family 3
• UniProt Protein Name: Ras association domain-containing protein 3
• Protein Family: Ras association domain-containing protein
• UniProt Gene Name: RASSF3
• UniProt Entry Name: RASF3_HUMAN

NCBI Description

The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.[supplied by OMIM, Jul 2003]

Uniprot Description

RASSF3: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q14.2

Cellular Component: microtubule; cytoplasm; plasma membrane

Molecular Function: identical protein binding; protein binding

Biological Process: signal transduction