Rat selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit | SELP elisa kit

Rat P-Selectin ELISA Kit

Cat. Num. AAA702140

• Gene Names: Selp; PSELECT
• Reactivity: Rat
• Synonyms: selectin P (granule membrane protein 140kDa; antigen CD62); Rat P-Selectin ELISA Kit; Rat P-Selectin ELISA kit; RP1-86F14.2; CD62; CD62P; FLJ45155; GMP140; GRMP; LECAM3; PADGEM; PSEL; OTTHUMP00000032609; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet alpha-granule membrane; SELP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat SELP. No significant cross-reactivity or interference between Rat SELP and analogues was observed.
• Sequence Length: 768

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 9.38 ng/ml–600 ng/ml
• Sensitivity: 2.34ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cell Adhesion
• Protein Biological Process 3: Cell adhesion
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SELP elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P-Selectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for P-Selectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain P-Selectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of P-Selectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6981524
• NCBI GeneID: 25651
• NCBI Accession #: NP_037246.1
• NCBI GenBank Nucleotide #: NM_013114.1
• UniProt Accession #: P98106
• Molecular Weight: 83,517 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin P
• NCBI Official Symbol: Selp
• NCBI Official Synonym Symbols: PSELECT
• NCBI Protein Information: P-selectin; LECAM3; PADGEM; GMP-140; selectin, platelet; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: Selp
• UniProt Synonym Gene Names: GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_RAT

NCBI Description

mediates leukocyte interaction with platelets and endothelial binding after vascular injury; may play a role in inflammatory response [RGD, Feb 2006]

Uniprot Description

Function: Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1