Typical Testing Data/Standard Curve (for reference only)
Human Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit | HSP90b1 elisa kit
Human Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit
Gene Names
HSP90B1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
Reactivity
Human
Synonyms
Heat Shock Protein 90kDa Beta 1 (HSP90b1); Human Heat Shock Protein 90kDa Beta 1 (HSP90b1) ELISA Kit; HSP90b1 elisa kit
Reactivity
Human
Sequence Length
315
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.1ng/ml-40ng/ml
Sensitivity
0.055ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HSP90b1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Heat Shock 70kDa Protein 1A (also known as HSPA1A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human HSPA1A antibody. HSPA1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human HSPA1A Antibody is added and binds to HSPA1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPA1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human HSPA1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human HSPA1A antibody. HSPA1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human HSPA1A Antibody is added and binds to HSPA1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPA1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human HSPA1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
92,469 Da
NCBI Official Full Name
HSP90B1 protein
NCBI Official Synonym Full Names
heat shock protein 90 beta family member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m
NCBI Protein Information
endoplasmin
UniProt Protein Name
Endoplasmin
Protein Family
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
GRP94; TRA1; GRP-94
NCBI Description
This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
Uniprot Description
Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Research Articles on HSP90b1
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Product Notes
The Human HSP90b1 hsp90b1 (Catalog #AAA162640) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA162640 ELISA Kit recognizes Human HSP90b1. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 90kDa Beta 1 (HSP90b1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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