Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat P-Selectin (SELP) Standard Curve.)
Rat P-Selectin (SELP) ELISA Kit | SELP elisa kit
Rat P-Selectin (SELP) ELISA Kit
Gene Names
Selp; PSELECT
Reactivity
Rat
Synonyms
P-Selectin (SELP); Rat P-Selectin (SELP) ELISA Kit; SELP; RP1-86F14.2; CD62; CD62P; FLJ45155; GMP140; GRMP; LECAM3; PADGEM; PSEL; OTTHUMP00000032609; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet alpha-granule membrane; SELP elisa kit
Reactivity
Rat
Specificity
Rat P-Selectin (SELP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SELP. No significant cross-reactivity or interference between Rat SELP and analogues was observed.
Sequence Length
768
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat P-Selectin (SELP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat P-Selectin (SELP) Standard Curve.)
Related Product Information for SELP elisa kit
Description: This Rat P-Selectin (SELP) ELISA Kit employs a two-site sandwich ELISA to quantitate SELP in samples. An antibody specific for SELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: P-selectin is the largest of the known selectins at 140kDa. It contains nine consensus repeats (CR) and extends approximately 40 nm from the endothelial surface. P-selectin is expressed in a-granules of activated platelets and granules of endothelial cells. Within minutes of stimulation of the endothelial cells by inflammatory mediators such as histamine, thrombin, or phorbol esters, P-selectin is surface-expressed. Expression of P-selectin also occurs from the surgical trauma endured during preparation of the tissues for intravital microscopy. The expression is short-lived, reaching its peak after only ten minutes. Additional synthesis of P-selectin is brought about within two hours by cytokines such as interleukin-1 (IL-1) or tumor necrosis factor a (TNF-a).
Background: P-selectin is the largest of the known selectins at 140kDa. It contains nine consensus repeats (CR) and extends approximately 40 nm from the endothelial surface. P-selectin is expressed in a-granules of activated platelets and granules of endothelial cells. Within minutes of stimulation of the endothelial cells by inflammatory mediators such as histamine, thrombin, or phorbol esters, P-selectin is surface-expressed. Expression of P-selectin also occurs from the surgical trauma endured during preparation of the tissues for intravital microscopy. The expression is short-lived, reaching its peak after only ten minutes. Additional synthesis of P-selectin is brought about within two hours by cytokines such as interleukin-1 (IL-1) or tumor necrosis factor a (TNF-a).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
P-selectin
NCBI Official Synonym Full Names
selectin P
NCBI Official Symbol
Selp
NCBI Official Synonym Symbols
PSELECT
NCBI Protein Information
P-selectin
UniProt Protein Name
P-selectin
Protein Family
UniProt Gene Name
Selp
UniProt Synonym Gene Names
GMP-140; LECAM3; PADGEM
NCBI Description
mediates leukocyte interaction with platelets and endothelial binding after vascular injury; may play a role in inflammatory response [RGD, Feb 2006]
Uniprot Description
Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1 ().
Research Articles on SELP
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Product Notes
The Rat SELP selp (Catalog #AAA9711899) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711899 ELISA Kit recognizes Rat SELP. It is sometimes possible for the material contained within the vial of "P-Selectin (SELP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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