Human CD62P ELISA Kit | SELP elisa kit

Human CD62P ELISA Kit

Cat. Num. AAA8244696

• Gene Names: SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• Reactivity: Human
• Applications: ELISA
• Synonyms: CD62P; Human CD62P ELISA Kit; GMRP; GRMP; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; SELP elisa kit

• Reactivity: Human
• Specificity: The Human CD62P ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD62P proteins within the range of 125 pg/ml - 8000 pg/ml.
• Sequence Length: 830

• Applicable Applications for SELP elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 30 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for SELP elisa kit

Principle of the Assay||The Human CD62P ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62P in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD62P coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62P present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62P antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62P bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).

NCBI and Uniprot Product Information

• NCBI GI #: 215274139
• NCBI GeneID: 6403
• UniProt Accession #: P16109; Q5R344; Q8IVD1
• Molecular Weight: 90,834 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin P
• NCBI Official Symbol: SELP
• NCBI Official Synonym Symbols: CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• NCBI Protein Information: P-selectin
• UniProt Protein Name: P-selectin
• Protein Family: P-selectin
• UniProt Gene Name: SELP
• UniProt Synonym Gene Names: GMRP; GRMP; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_HUMAN

NCBI Description

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; integral to plasma membrane; nucleoplasm; plasma membrane; platelet alpha granule membrane; platelet dense granule membrane

Molecular Function: calcium-dependent protein binding; fucose binding; glycoprotein binding; glycosphingolipid binding; heparin binding; lipopolysaccharide binding; protein binding; sialic acid binding

Biological Process: blood coagulation; cell adhesion; defense response to Gram-negative bacterium; heterophilic cell adhesion; inflammatory response; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; platelet activation; platelet degranulation; positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of phosphoinositide 3-kinase cascade; regulation of cellular extravasation; regulation of integrin activation; response to lipopolysaccharide; response to organic cyclic substance

Disease: Ige Responsiveness, Atopic