Human Ephrin Type A Receptor 1 ELISA Kit | EPHA1 elisa kit

Human Ephrin Type A Receptor 1 ELISA Kit

Cat. Num. AAA762420

• Gene Names: EPHA1; EPH; EPHT; EPHT1
• Reactivity: Human
• Synonyms: Ephrin Type A Receptor 1; Human Ephrin Type A Receptor 1 ELISA Kit; EPHA1/Esk/EC 2.7.10/EC 2.7.10.1/EPH receptor A1/eph tyrosine kinase 1/EphA1/EPHT/EPHT1erythropoietin-producing hepatoma amplified sequence/MGC163163/oncogene EPH/soluble EPHA1 variant 1/soluble EPHA1 variant 2/Tyrosine-protein kinase receptor EPH; EPHA1 elisa kit

• Reactivity: Human
• Sequence Length: 976

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EPHA1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EPHA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EPHA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EPHA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human EPHA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 221316650
• NCBI GeneID: 2041
• NCBI Accession #: NP_005223.4
• NCBI GenBank Nucleotide #: NM_005232.4
• Molecular Weight: 51,069 Da
• NCBI Official Full Name: ephrin type-A receptor 1
• NCBI Official Synonym Full Names: EPH receptor A1
• NCBI Official Symbol: EPHA1
• NCBI Official Synonym Symbols: EPH; EPHT; EPHT1
• NCBI Protein Information: ephrin type-A receptor 1
• UniProt Protein Name: Ephrin type-A receptor 1
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA1
• UniProt Synonym Gene Names: EPH; EPHT; EPHT1; hEpha1
• UniProt Entry Name: EPHA1_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA1: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Misexpressed in several cancers, including upregulation in head and neck cancer, and downregulation in invasive breast cancer cell lines and glioblastoma

Protein type: Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; protein kinase activity; protein kinase binding; transmembrane-ephrin receptor activity

Biological Process: angiogenesis; axon guidance; cell surface receptor linked signal transduction; ephrin receptor signaling pathway; negative regulation of cell migration; negative regulation of protein kinase activity; peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of stress fiber formation; protein amino acid autophosphorylation; regulation of GTPase activity; somatic stem cell maintenance