Mouse SQSTM1 Monoclonal Antibody | anti-SQSTM1 antibody

SQSTM1 (sequestosome 1, A170, OSIL, PDB3, ZIP3, p60, p62, p62B) (AP)

Cat. Num. AAA6163813

• Gene Names: SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
• Applications: Western Blot
• Purity: Purified
• Synonyms: SQSTM1; Monoclonal Antibody; SQSTM1 (sequestosome 1; A170; OSIL; PDB3; ZIP3; p60; p62; p62B) (AP); sequestosome 1; p62B; anti-SQSTM1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 1C9
• Specificity: Recognizes SQSTM1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 440

• Applicable Applications for anti-SQSTM1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: SQSTM1 (AAH03139.1, 1aa-440aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged SQSTM1 is approximately 3ng/ml as a capture antibody.)

Related Product Information for anti-SQSTM1 antibody

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Product Categories/Family for anti-SQSTM1 antibody

Antibodies; Ubiquitin

References

1. Hypoxia counteracts taxol-induced apoptosis in MDA-MB-231 breast cancer cells: role of autophagy and JNK activation.Notte A, Ninane N, Arnould T, Michiels CCell Death Dis. 2013 May 16;4:e638. doi: 10.1038/cddis.2013.167. 2.BNIP3 protects HepG2 cells against etoposide-induced cell death under hypoxia by an autophagy-independent pathway. Cosse JP, Rommelaere G, Ninane N, Arnould T, Michiels C.Biochem Pharmacol. 2010 Jul 15. 3.A Role for NBR1 in Autophagosomal Degradation of Ubiquitinated Substrates.Kirkin V, Lamark T, Sou YS, Bjorkoy G, Nunn JL, Bruun JA, Shvets E, McEwan DG, Clausen TH, Wild P, Bilusic I, Theurillat JP, Overvatn A, Ishii T, Elazar Z, Komatsu M, Dikic I, Johansen T.Mol Cell. 2009 Feb 27;33(4):505-16.

NCBI and Uniprot Product Information

• NCBI GI #: 13111937
• NCBI GeneID: 8878
• NCBI Official Full Name: Sequestosome 1
• NCBI Official Synonym Full Names: sequestosome 1
• NCBI Official Symbol: SQSTM1
• NCBI Official Synonym Symbols: p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
• NCBI Protein Information: sequestosome-1
• Protein Family: Sequestosome

NCBI Description

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]