Typical Testing Data/Standard Curve (for reference only)
(Rat sL-Selectin PicoKine ELISA Kit standard curve )
Rat sL-Selectin ELISA Kit | SELL elisa kit
Rat sL-Selectin PicoKine ELISA Kit
Gene Names
Sell; A.11; LECAM-1; L-selectin
Reactivity
Rat
Synonyms
sL-Selectin; Rat sL-Selectin PicoKine ELISA Kit; L-selectin; selectin L; A.11; AI528707; CD62 antigen ligand; CD62 antigen-like family member L; CD62L antigen; gp90 MEL; hLHRc; IgA nephropathy; susceptibility to; included; L Selectin; L selectin precursor; LAM 1; LAM1; LECAM1; Leu 8; Leu8; Leukocyte adhesion molecule 1; Leukocyte endothelial cell adhesion molecule 1; Leukocyte surface antigen Leu 8; Leukocyte surface antigen Leu8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; Ly-22; Ly-m22; Lyam 1; LYAM1; Lymph node homing receptor; Lymphocyte adhesion molecule 1; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; PLNHR; Selectin L; Selectin L (lymphocyte adhesion molecule 1); Selectin; lymphocyte; SELL; TQ1; SELL elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat sL-Selectin
Sequence Length
372
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
625pg/ml-40,000pg/ml
Sensitivity
< 20pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat sL-Selectin PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat sL-Selectin PicoKine ELISA Kit standard curve )
Related Product Information for SELL elisa kit
Principle of the assay: rat L-Selectin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for L-Selectin has been precoated onto 96-well plates. Standards(NSO, W39-N332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for L-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat L-Selectin amount of sample captured in plate.
Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
Background: L-selectin, also known as CD62L, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17.SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.1 L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42,471 Da
NCBI Official Full Name
Lymphocyte membrane protein A.11
NCBI Official Synonym Full Names
selectin L
NCBI Official Symbol
Sell
NCBI Official Synonym Symbols
A.11; LECAM-1; L-selectin
NCBI Protein Information
L-selectin; LAM-1; ly-22; LECAM1; selectin, lymphocyte; lymphocyte antigen 22; lymph node homing receptor; leukocyte adhesion molecule 1; CD62 antigen-like family member L; lymphocyte surface MEL-14 antigen; leukocyte-endothelial cell adhesion molecule 1
UniProt Protein Name
L-selectin
Protein Family
UniProt Gene Name
Sell
UniProt Entry Name
Q63762_RAT
NCBI Description
lymphocyte adhesion molecule that facilitates adhesion of lymphocytes to high endothelial venules [RGD, Feb 2006]
Uniprot Description
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Research Articles on SELL
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Product Notes
The Rat SELL sell (Catalog #AAA176513) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176513 ELISA Kit recognizes Rat SELL. It is sometimes possible for the material contained within the vial of "sL-Selectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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