CD62L Polyclonal Antibody | anti-CD62L antibody

Anti-CD62L Antibody

Cat. Num. AAA177770

• Gene Names: SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Immunogen Affinity Purified
• Synonyms: CD62L; Polyclonal Antibody; Anti-CD62L Antibody; L-selectin; A.11; AI528707; CD62 antigen ligand; CD62 antigen-like family member L; gp90 MEL; gp90-MEL; hLHRc; IgA nephropathy; susceptibility to; included; L Selectin; LAM-1; LAM1; LECAM1; LEU8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte surface antigen Leu8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LSEL; Ly-22; Ly-m22; Lyam-1; LYAM1; LYAM1_HUMAN; Lymph node homing receptor; Lymphocyte adhesion molecule 1; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; MEL-14; Pln homing receptor; PLNHR; Selectin L; Selectin; lymphocyte; SELL; TQ1; selectin L; anti-CD62L antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 385

• Applicable Applications for anti-CD62L antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: ELISA Concentration: 0.1-0.5ug/ml; Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human CD62L recombinant protein (Position: W39-Y372). Human CD62L shares 77.5 % and 78.7 % amino acid (aa) sequence identity with mouse and rat CD62L, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti- L-selectin Picoband antibody, MBS177770, IHC(P)IHC(P): Human Tonsil Tissue )

Western Blot (WB)

(Anti- L-selectin Picoband antibody, MBS177770, Western blottingAll lanes: Anti L-selectin (MBS177770) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: JURKAT Whole Cell Lysate at 40ugLane 3: CEM Whole Cell Lysate at 40ugLane 4: HL60 Whole Cell Lysate at 40ugLane 5: Rat Spleen Tissue Lysate at 50ugLane 6: Mouse Spleen Tissue Lysate at 50ugPredicted bind size: 42KDObserved bind size: 42KD)

Related Product Information for anti-CD62L antibody

Description: Rabbit IgG polyclonal antibody for L-selectin(SELL) detection. Tested with WB, IHC-P, ELISA in Human;Mouse;Rat.

Background: CD62L, also known as L-selectin, is a cell adhesion molecule found on leukocytes. It belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups. It is cleaved by ADAM17. SELL (L-selectin) is a cell surface component that is a member of a family of adhesion/homing receptors which play important roles in leukocyte-endothelial cell interactions. The molecule is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.

References

1. "Entrez Gene: SELL selectin L (lymphocyte adhesion molecule 1)". 2. Cotran; Kumar, Collins (1998). Robbins Pathologic Basis of Disease. Philadelphia: W.B Saunders Company.

NCBI and Uniprot Product Information

• NCBI GI #: 262206315
• NCBI GeneID: 6402
• NCBI Accession #: NP_000646.2
• NCBI GenBank Nucleotide #: NM_000655.4
• UniProt Accession #: P14151; P15023; Q9UJ43; B2R6Q8
• Molecular Weight: 43,618 Da
• NCBI Official Full Name: L-selectin
• NCBI Official Synonym Full Names: selectin L
• NCBI Official Symbol: SELL
• NCBI Official Synonym Symbols: TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• NCBI Protein Information: L-selectin
• UniProt Protein Name: L-selectin
• UniProt Gene Name: SELL
• UniProt Synonym Gene Names: LNHR; LYAM1; LAM-1; LECAM1
• UniProt Entry Name: LYAM1_HUMAN

NCBI Description

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23-q25

Cellular Component: external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: carbohydrate binding; cell adhesion molecule binding; glycosphingolipid binding; heparin binding; protease binding; protein binding

Biological Process: cell adhesion; leukocyte migration; regulation of immune response; response to ATP

Disease: Iga Nephropathy, Susceptibility To, 1