L-selectin Recombinant Protein | SELL recombinant protein

L-selectin Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120993

• Gene Names: SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: L-selectin; L-selectin Protein; Human; Recombinant (His Tag); Human CD62L/L-Selectin/SELL Protein (His Tag); CD62L Protein; LAM1 Protein; LECAM1 Protein; LEU8 Protein; LNHR Protein; LSEL Protein; LYAM1 Protein; PLNHR Protein; TQ1 Protein; selectin L; SELL recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn332

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp 39
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human SELL (P14151-1) extracellular domain (Met 1-Asn 332) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SELL recombinant protein

Background: L-selectin (SELL), also known as CD62L, is a key adhesion molecule that regulates both the migration of leukocytes at sites of inflammation and the recirculation of lymphocytes between blood and lymphoid tissues. It belongs to the selectin family of proteins, and consisting of a large, highly glycosylated, extracellular domain, a single spanning transmembrane domain and a small cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin-mediated lymphocyte recirculation is required for maintaining the appropriate tissue distribution of lymphocyte subpopulations including naa¯ve and effector subsets such as regulatory T cells. In addition, L-selectin-mediated entry into peripheral lymph nodes is required for optimal induction of lymphocyte homeostatic proliferation during lymphopenia. Importantly, L-selectin has been shown to have both adhesive and signaling functions during leukocyte migration. L-selectin has also been shown to mediate leukocyte recruitment during chronic inflammatory and autoimmune diseases and thus is a potential therapeutic target for drug development.

References

Smalley DM, et al. (2005) L-selectin: mechanisms and physiological significance of ectodomain cleavage. J Cell Mol Med. 9(2): 255-66.Grailer JJ, et al. (2009) L-selectin: role in regulating homeostasis and cutaneous inflammation. J Dermatol Sci. 56(3): 141-7.

NCBI and Uniprot Product Information

• NCBI GI #: 262206315
• NCBI GeneID: 6402
• NCBI Accession #: NP_000646.2
• NCBI GenBank Nucleotide #: NM_000655.4
• UniProt Accession #: P14151; P15023; Q9UJ43; B2R6Q8
• Molecular Weight: 42,187 Da
• NCBI Official Full Name: L-selectin
• NCBI Official Synonym Full Names: selectin L
• NCBI Official Symbol: SELL
• NCBI Official Synonym Symbols: TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
• NCBI Protein Information: L-selectin; gp90-MEL; pln homing receptor; lymph node homing receptor; lymphocyte adhesion molecule 1; leukocyte surface antigen Leu-8; CD62 antigen-like family member L; leukocyte-endothelial cell adhesion molecule 1
• UniProt Protein Name: L-selectin
• Protein Family: L-selectin
• UniProt Gene Name: SELL
• UniProt Synonym Gene Names: LNHR; LYAM1; LAM-1; LECAM1
• UniProt Entry Name: LYAM1_HUMAN

NCBI Description

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23-q25

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; protease binding; cell adhesion molecule binding; carbohydrate binding; glycosphingolipid binding

Biological Process: regulation of immune response; cell adhesion; blood coagulation; leukocyte migration; response to ATP

Disease: Iga Nephropathy, Susceptibility To, 1