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Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

5940 results for " Nucleus membrane" - showing 400-450

Western Blot (WB) (Western blot analysis of lysates from A431,Raji cell line (from left to right), using LYN Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

LYN, Monoclonal Antibody (Cat# AAA9231837)

Full Name
LYN Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human
Applications
WB, EIA
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells using MTUS1 antibody. Blue: DAPI for nuclear staining.)

MTUS1, Polyclonal Antibody (Cat# AAA9133478)

Full Name
MTUS1 Polyclonal Antibody
Gene Names
MTUS1; ATBP; ATIP; ICIS; MP44; ATIP3; MTSG1
Reactivity
Human
Applications
IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, using TESC Antibody. The lane on the left was treated with blocking peptide.)

TESC, Polyclonal Antibody (Cat# AAA9611520)

Full Name
TESC Antibody
Gene Names
TESC; TSC; CHP3
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Rabbit, Dog
Applications
WB
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of RYK using K562 whole cell lysates)

RYK, Polyclonal Antibody (Cat# AAA9608761)

Full Name
RYK Antibody
Gene Names
RYK; JTK5; RYK1; JTK5A; D3S3195
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of Jurkat cells, using Phospho-NDRG1-T346 antibody at 1:2000 dilution. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1s.)

NDRG1-T346, Polyclonal Antibody (Cat# AAA9135645)

Full Name
Phospho-NDRG1-T346 pAb
Gene Names
NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB) (Western blot analysis of GPR81 expression in 293 cell line lysate ;, The lane on the left is treated with the antigen-specific peptide.)

GPR81, Polyclonal Antibody (Cat# AAA9604280)

Full Name
GPR81 Antibody
Gene Names
HCAR1; HCA1; GPR81; LACR1; FKSG80; GPR104; TAGPCR; TA-GPCR
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601724 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

PKCB, Polyclonal Antibody (Cat# AAA9601724)

Full Name
PKCB Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9603682 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

PIP5K1A, Polyclonal Antibody (Cat# AAA9603682)

Full Name
PIP5K1A Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600868 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

PKCB, Polyclonal Antibody (Cat# AAA9600868)

Full Name
Phospho-PKCB (Ser661) Antibody
Gene Names
PRKCB; PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9606759 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

AKT2, Polyclonal Antibody (Cat# AAA9606759)

Full Name
AKT2 Antibody
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of 293T whole cell lysates, using SIGMAR1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

SIGMAR1, Polyclonal Antibody (Cat# AAA9608000)

Full Name
SIGMAR1 Antibody
Gene Names
SIGMAR1; SRBP; ALS16; DSMA2; OPRS1; SR-BP; SIG-1R; SR-BP1; sigma1R; hSigmaR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of Synuclein phosphorylation expression in Mouse brain tissue lysates, The lane on the left is treated with the antigen-specific peptide.)

Synuclein, Polyclonal Antibody (Cat# AAA9601056)

Full Name
Phospho-Synuclein (Ser129) Antibody
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600930 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

FGFR1, Polyclonal Antibody (Cat# AAA9600930)

Full Name
Phospho-FGFR1 (Tyr154) Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9605392 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CLIC4, Polyclonal Antibody (Cat# AAA9605392)

Full Name
CLIC4 Antibody
Gene Names
CLIC4; H1; huH1; p64H1; CLIC4L; MTCLIC
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600819 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

EGFR, Polyclonal Antibody (Cat# AAA9600819)

Full Name
Phospho-EGFR (Tyr1172) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of APP in lysates of HeLa, using APP Antibody.)

APP, Polyclonal Antibody (Cat# AAA9610975)

Full Name
APP Antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human, Mouse, Rat
Predicted: Pig, Horse, Chicken, Xenopus
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601994 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

HER4, Polyclonal Antibody (Cat# AAA9601994)

Full Name
HER4 Antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) ( All lanes : Anti-RAF1 Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

RAF1, Polyclonal Antibody (Cat# AAA9217155)

Full Name
RAF1 Antibody (S233)
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human
Applications
WB, IHC-P, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using S100P antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

S100P, Polyclonal Antibody (Cat# AAA9134772)

Full Name
S100P Polyclonal Antibody
Gene Names
S100P; MIG9
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis NUP155 using COS7 whole cell lysates)

NUP155, Polyclonal Antibody (Cat# AAA9610025)

Full Name
NUP155 Antibody
Gene Names
NUP155; N155; ATFB15
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Figure 1: Western blot analysis using CA9 mouse mAb against Hela (1) and A549 (2) cell lysate.)

CA9, Monoclonal Antibody (Cat# AAA9602596)

Full Name
CA9 Antibody
Gene Names
CA9; MN; CAIX
Reactivity
Human
Applications
WB, IHC, EIA, FC/FACS
Purity
Affinity-Chromatography
Pricing
$260/0.05 mL | $325/0.1 mL | $400/0.2 mL | $1,750/5x0.2 mL
Western Blot (WB) (Figure 1: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysate.)

SIRT1, Monoclonal Antibody (Cat# AAA9602450)

Full Name
SIRT1 Antibody
Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Human, Rat, Monkey
Applications
WB, IHC, IF, ICC, EIA, FC/FACS
Purity
Affinity-Chromatography
Pricing
$260/0.05 mL | $325/0.1 mL | $400/0.2 mL | $1,750/5x0.2 mL
Immunohistochemistry (IHC) (MBS9603371 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

ARL3, Polyclonal Antibody (Cat# AAA9603371)

Full Name
ARL3 Antibody
Gene Names
ARL3; ARFL3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Activity Data (Figure. The binding activity of CFL1 with ACTb.Cofilin 1 (non-muscle; n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. Besides, Actin Beta (ACTb) has been identified as an interactor of CFL1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CFL1 and recombinant human ACTb. Briefly, CFL1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CFL1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CFL1 and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)

Cofilin 1, Non Muscle (CFL1), Active Protein (Cat# AAA2105143)

Full Name
Active Cofilin 1, Non Muscle (CFL1)
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$185/0.01 mg | $360/0.05 mg | $550/0.1 mg | $670/0.2 mg | $1,300/0.5 mg | $1,935/1 mg | $4,750/5 mg
Western Blot (WB) (Anti-RAF1 Antibody at 1:1000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

RAF1, Polyclonal Antibody (Cat# AAA9217154)

Full Name
RAF1 Antibody (S621)
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human; Predicted Reactivity: Bovine, Chicken, Mouse, Rat
Applications
WB, IHC, EIA
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) (MBS9601665 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

SGK, Polyclonal Antibody (Cat# AAA9601665)

Full Name
SGK Antibody
Gene Names
SGK1; SGK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)

Alpha Synuclein, Monoclonal Antibody (Cat# AAA808931)

Full Name
Alpha Synuclein Antibody, Clone 3C11: PerCP
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse; Rat
Applications
WB, DB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB) (Western blot analysis on 293 cell lysate using Phospho-AKT1(Thr308) Antibody. The lane on the left is treated with the antigen-specific peptide.)

AKT1, Polyclonal Antibody (Cat# AAA9600582)

Full Name
Phospho-AKT1(Thr308) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601078 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

mTOR, Polyclonal Antibody (Cat# AAA9601078)

Full Name
Phospho-mTOR (Ser2481) Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Figure 1. Western blot analysis of NADPH oxidase 4 using anti-NADPH oxidase 4 antibody (MBS1750390).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human 293T whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NADPH oxidase 4 at approximately 85KD. The expected band size for NADPH oxidase 4 is at 67KD. )

NADPH oxidase 4, Polyclonal Antibody (Cat# AAA1750390)

Full Name
Anti-NADPH oxidase 4 Picoband antibody
Gene Names
NOX4; KOX; KOX-1; RENOX
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF) (MBS9601699 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

EGFR, Polyclonal Antibody (Cat# AAA9601699)

Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of CKIP-1 Antibody (N-term) in 293 cell line lysates (35ug/lane). CKIP-1 (arrow) was detected using the purified Pab.)

CKIP-1, Polyclonal Antibody (Cat# AAA9207531)

Full Name
CKIP-1 Antibody (N-term)
Gene Names
PLEKHO1; JBP; CKIP1; OC120; CKIP-1
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of PDK1 in lysates of HepG2?, using PDK1 Antibody.)

PDK1, Polyclonal Antibody (Cat# AAA9610903)

Full Name
PDK1 Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Dog, Chicken
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Western Blot (WB) (Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.)

AKT2, Monoclonal Antibody (Cat# AAA9602803)

Full Name
AKT2 Antibody
Gene Names
AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
Reactivity
Human, Rat, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
Affinity-Chromatography
Pricing
$260/0.05 mL | $325/0.1 mL | $400/0.2 mL | $1,750/5x0.2 mL
Immunohistochemistry (IHC) (MBS9607993 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

LBR, Polyclonal Antibody (Cat# AAA9607993)

Full Name
LBR Antibody
Gene Names
LBR; PHA; LMN2R; PHASK; TDRD18; DHCR14B
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis on mouse brain cell lysate using Alpha-synuclein Antibody, The lane on the left is treated with the antigen-specific peptide.)

Alpha-synuclein, Polyclonal Antibody (Cat# AAA9600319)

Full Name
Alpha-synuclein Antibody
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of human brain, using RGS2 antibody. The lane on the left is treated with the antigen-specific peptide.)

RGS2, Polyclonal Antibody (Cat# AAA9607277)

Full Name
RGS2 Antibody
Gene Names
RGS2; G0S8
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9606894 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

MAGED1, Polyclonal Antibody (Cat# AAA9606894)

Full Name
MAGED1 Antibody
Gene Names
MAGED1; NRAGE; DLXIN-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of GSK3B expression in various lysates)

GSK3A/B, Polyclonal Antibody (Cat# AAA9607461)

Full Name
GSK3A/B Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of mouse liver tissue, using VPS37A antibody.)

VPS37A, Polyclonal Antibody (Cat# AAA9603471)

Full Name
VPS37A Antibody
Gene Names
VPS37A; HCRP1; PQBP2; SPG53
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9602019 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

SirT1, Polyclonal Antibody (Cat# AAA9602019)

Full Name
SirT1 Antibody
Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of Hela whole cell lysates, using NDRG1 Antibody. The lane on the left is treated with the antigen-specific peptide.)

NDRG1, Polyclonal Antibody (Cat# AAA9607518)

Full Name
NDRG1 Antibody
Gene Names
NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601930 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Smad3, Polyclonal Antibody (Cat# AAA9601930)

Full Name
Smad3 Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Figure 5. IHC analysis of Ran using anti-Ran antibody (MBS1750350). Ran was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Ran Antibody (MBS1750350) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

Ran, Polyclonal Antibody (Cat# AAA1750350)

Full Name
Anti-Ran Picoband Antibody
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of mouse brain, using SPRED1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

SPRED1, Polyclonal Antibody (Cat# AAA9134961)

Full Name
SPRED1 Polyclonal Antibody
Gene Names
SPRED1; NFLS; hSpred1; spred-1; PPP1R147
Reactivity
Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$225/0.05 mL | $305/0.1 mL | $465/0.2 mL
Western Blot (WB) (Western blot analysis of extracts of rat thymus, using TXK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)

TXK, Polyclonal Antibody (Cat# AAA9134784)

Full Name
TXK Polyclonal Antibody
Gene Names
TXK; RLK; TKL; BTKL; PTK4; PSCTK5
Reactivity
Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis UBE2A/B using LOVO whole cell lysates)

UBE2A/B, Polyclonal Antibody (Cat# AAA9610313)

Full Name
UBE2A/B Antibody
Gene Names
UBE2B; HR6B; UBC2; HHR6B; RAD6B; E2-17kDa
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of NOTCH2 (Cleaved-Ala1734) expression in Jurkat cells, treated with etoposide, The lane on the left is treated with the antigen-specific peptide.)

NOTCH2, Polyclonal Antibody (Cat# AAA9601428)

Full Name
NOTCH2 (Cleaved-Ala1734) Antibody
Gene Names
NOTCH2; hN2; AGS2; HJCYS
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody, Clone S3-26. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Slo2.2 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining around nucleus and some in cytoplasm.)

Slo2.2, Monoclonal Antibody (Cat# AAA800050)

Full Name
Slo2.2 Antibody: FITC
Gene Names
Kcnt1; Slack; rSlo2
Reactivity
Human (weak), Mouse, Rat
Applications
WB, IHC
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB) (Western blot analysis of PAFAH1B1 using HeLa whole cell lysates)

PAFAH1B1, Polyclonal Antibody (Cat# AAA9608558)

Full Name
PAFAH1B1 Antibody
Gene Names
PAFAH1B1; MDS; LIS1; LIS2; MDCR; NudF; PAFAH
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

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