Rabbit ARL3 Polyclonal Antibody | anti-ARL3 antibody

ARL3 Antibody

Cat. Num. AAA9603371

• Gene Names: ARL3; ARFL3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ARL3; Polyclonal Antibody; ARL3 Antibody; ADP ribosylation factor like 3; ADP-ribosylation factor-like protein 3; Arf like 3; Arf like protein 3; ARFL3; ARL3_HUMAN; anti-ARL3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARL3 antibody detects endogenous levels of ARL3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 182

• Applicable Applications for anti-ARL3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human ARL3.
• Subcellular Location: Golgi Apparatus Membrane. Cytoplasm > Cytoskeleton > Spindle. Nucleus. Cytoplasm > Cytoskeleton > Centrosome. Cytoplasm. Cell projection > Cilium. Detected predominantly in the photoreceptor connecting cilium. Present on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.
• Tissue Specificity: Expressed in the retina. Strongly expressed in connecting cilium, the myoid region of the inner segments (IS) and in cone photoreceptors (at protein level).
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (86%), Sheep (100%), Rabbit (86%), Dog (100%), Chicken (86%), Xenopus (86%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9603371 at 1/100 staining Human liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of HeLa cells, using ARL3 antibody.)

Related Product Information for anti-ARL3 antibody

Function: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.
Subunit Structure: Interacts with RP2. The GTP-bound form interacts with PDE6D. Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with SYS1. The GTP-bound form interacts with ARL2BP and PDE6D. Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3. Microtubule-associated protein. Does not interact with TBCC. May interact with GOLGA4.
Similarity: Belongs to the small GTPase superfamily. Arf family.

NCBI and Uniprot Product Information

• NCBI GI #: 4757774
• NCBI GeneID: 403
• NCBI Accession #: NP_004302.1
• NCBI GenBank Nucleotide #: NM_004311.3
• UniProt Accession #: P36405; Q53X83; Q5JSM2; B2R6C7
• Molecular Weight: Observed: 20 kDa; Predicted: 21 kDa
• NCBI Official Full Name: ADP-ribosylation factor-like protein 3
• NCBI Official Synonym Full Names: ADP ribosylation factor like GTPase 3
• NCBI Official Symbol: ARL3
• NCBI Official Synonym Symbols: ARFL3
• NCBI Protein Information: ADP-ribosylation factor-like protein 3
• UniProt Protein Name: ADP-ribosylation factor-like protein 3
• Protein Family: ADP-ribosylation factor-like protein
• UniProt Gene Name: ARL3
• UniProt Synonym Gene Names: ARFL3

NCBI Description

ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008]

Uniprot Description

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit.