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Western Blot (WB) (All lanes : Anti-STK25 Antibody at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human STK25 Polyclonal Antibody | anti-STK25 antibody

STK25 Antibody

Gene Names
STK25; SOK1; YSK1
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
STK25; Polyclonal Antibody; STK25 Antibody; Serine/threonine-protein kinase 25; 2.7.11.1; Ste20-like kinase; Sterile 20/oxidant stress-response kinase 1; SOK-1; Ste20/oxidant stress response kinase 1; SOK1; YSK1; anti-STK25 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This STK25 antibody is generated from a rabbit immunized with a recombinant protein of human STK25.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
426
Applicable Applications for anti-STK25 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Type
Recombinant Protein
Antigen Source
Human
Cellular Location
Cytoplasm. Golgi apparatus. Note=Localizes to the Golgi apparatus
Tissue Location
Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-STK25 Antibody at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-STK25 Antibody at 1:2000 dilutionLane 1: A431 whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Human testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-STK25 antibody
Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.
Product Categories/Family for anti-STK25 antibody
References
Pombo C.M.,et al.EMBO J. 15:4537-4546 (1996). Osada S.,et al.Oncogene 14:2047-2057 (1997). Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45 (2004). Hillier L.W.,et al.Nature 434:724-731 (2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48112 Da
NCBI Official Full Name
serine/threonine-protein kinase 25 isoform 1
NCBI Official Synonym Full Names
serine/threonine kinase 25
NCBI Official Symbol
STK25
NCBI Official Synonym Symbols
SOK1; YSK1
NCBI Protein Information
serine/threonine-protein kinase 25
UniProt Protein Name
Serine/threonine-protein kinase 25
UniProt Gene Name
STK25
UniProt Synonym Gene Names
SOK1; YSK1; SOK-1; Ste20/oxidant stress response kinase 1

NCBI Description

This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

YSK1: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; YSK subfamily

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm; Golgi apparatus

Molecular Function: protein binding; protein homodimerization activity; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: establishment of Golgi localization; Golgi localization; positive regulation of stress-activated MAPK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; response to hydrogen peroxide; response to oxidative stress; signal transduction

Research Articles on STK25

Similar Products

Product Notes

The STK25 stk25 (Catalog #AAA9231096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STK25 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STK25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the STK25 stk25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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