Western Blot (WB) (PDE7B Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PDE7B antibody detected the PDE7B protein (arrow).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS1497384 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human epityphlon using MBS1497384 at dilution 1:100)
Structure
Western Blot (WB) (Western blot analysis PRKAR2B using A549 whole cell lysates)
Structure
Western Blot (WB) (PDE4C Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PDE4C antibody detected the PDE4C protein (arrow).)
Western Blot (WB) (PRKAR1B Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PRKAR1B antibody detected the PRKAR1B protein (arrow).)
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATF4 Antibody (D) Composite.)
Western Blot (WB) (Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells, using IPKA Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IPKA Antibody. The picture on the right is blocked with the synthesized peptide.)
Western Blot (WB) (Western blot analysis of CREB expression in A431 (A), NIH3T3 (B) whole cell lysates.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of CREB staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)