ATF6B sirna

ATF6B siRNA (Human)

Cat. Num. AAA8208593

• Gene Names: ATF6B; G13; CREBL1; CREB-RP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATF6B; ATF6B siRNA (Human); CREBL1; G13; Cyclic AMP-dependent transcription factor ATF-6 beta; cAMP-dependent transcription factor ATF-6 beta; Activating transcription factor 6 beta; ATF6-beta; Protein G13; cAMP response element-binding protein-related protein; Creb-rp; cAMP-responsive element-binding protein-like 1; ATF6B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATF6B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 700

• Applicable Applications for ATF6B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATF6B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATF6B sirna

siRNA to inhibit ATF6B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209954788
• NCBI GeneID: 1388
• NCBI Accession #: NP_001129625.1
• NCBI GenBank Nucleotide #: NM_001136153.1
• UniProt Accession #: Q99941; Q13269; Q14343; Q14345; Q5SSW7; Q99635; Q99637; Q9H3V9; Q9H3W1; Q9NPL0; B0UYX6
• Molecular Weight: 76,412 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-6 beta isoform b
• NCBI Official Synonym Full Names: activating transcription factor 6 beta
• NCBI Official Symbol: ATF6B
• NCBI Official Synonym Symbols: G13; CREBL1; CREB-RP
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-6 beta
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-6 beta
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF6B
• UniProt Synonym Gene Names: CREBL1; G13; cAMP-dependent transcription factor ATF-6 beta; ATF6-beta; Creb-rp
• UniProt Entry Name: ATF6B_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

ATF6B: Transcriptional factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN(9)CCAC[GA]-3') when NF-Y is bound to ERSE. Belongs to the bZIP family. ATF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; intracellular; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription, DNA-dependent; unfolded protein response; signal transduction