The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
PIAS1, CT E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1) (MaxLight 405)
PIAS1, NT (E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1) (MaxLight 405)
DDX3, CT (ATP-dependent RNA Helicase DDX3X, DEAD Box Protein 3, X-Chromosomal, Helicase-like Protein 2, HLP2, DEAD Box, X Isoform, DDX3X, DBX) (Biotin)
PIAS1, NT (E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1) (FITC)
Western Blot (WB) (Western blot analysis of DDX53 antibody (C-term) in mouse kidney tissue lysates (35ug/lane). DDX53 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of WiDr cells using DDX53 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of DDX58 in 293 cell line lysates (35ug/lane). DDX58 (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of DDX58 in 293 cell line lysates (35ug/lane). DDX58 (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western Blot detection against Immunogen (36.89kD).)
Western Blot (WB) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
Western Blot (WB) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Western Blot (WB) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
Western Blot (WB) (Western blot analysis of DDX39 Antibody (Center Y265) in HepG2 cell line lysates (35ug/lane). DDX39 (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of DDX39 (arrow) using rabbit polyclonal DDX39 Antibody (Center Y265). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DDX39 gene.)
Western Blot (WB) (Western blot analysis of anti-DDX4 Antibody (C-term) (RB13974) in K562 cell line lysates (35ug/lane). DDX4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of anti-DDX4 Antibody (C-term) (RB13974) in K562 cell line lysates (35ug/lane). DDX4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western Blot detection against Immunogen (37.55kD).)
Western Blot (WB) (Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody. Lane 1: DDX54 transfected lysate (98.6kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml].)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged DDX54 is 0.03ng/ml as a capture antibody.)
Western Blot (WB) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)
Western Blot (WB) (Western Blot detection against Immunogen (36.89kD).)
Western Blot (WB) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
Western Blot (WB) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Western Blot (WB) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cell using 211414. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western Blot detection against Immunogen (35.64kD).)
Western Blot (WB) (DHX9 monoclonal antibody Western Blot analysis of DHX9 expression in HeLa NE.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to DHX9 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to DHX9 on HeLa cell. [antibody concentration 10ug/ml])
Western Blot (WB) (Western blot analysis of anti-DDX4 Antibody (C-term) (RB13974) in K562 cell line lysates (35ug/lane). DDX4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of anti-DDX4 Antibody (C-term) (RB13974) in K562 cell line lysates (35ug/lane). DDX4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cell using 211414. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cell using 211414. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Lanes:Lane 1: Untreated human U251 lysateLane 2: IL-1beta treated human U251 lysatePrimary Antibody Dilution:1:500Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:500Gene Name:PIAS1Submitted by:Anonymous)
Western Blot (WB) (WB Suggested Anti-PIAS1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: COLO205 cell lysateThere is BioGPS gene expression data showing that PIAS1 is expressed in COLO205)
Western Blot (WB) (Western blot analysis of DDX5 antibody (Center) in Romas cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain with DDX5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western Blot detection against Immunogen (37.11kD).)
Western Blot (WB) (DDX41 monoclonal antibody, Western Blot analysis of DDX41 expression in HeLa NE.)
Western Blot (WB) (Western Blot analysis of DDX41 expression in transfected 293T cell line by DDX41 monoclonal antibody. Lane 1: DDX41 transfected lysate (7kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to DDX41 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged DDX41 is ~0.3ng/ml as a capture antibody.)
Western Blot (WB) (Western Blot detection against Immunogen (37.11kD).)
Western Blot (WB) (DDX41 monoclonal antibody, Western Blot analysis of DDX41 expression in HeLa NE.)
Western Blot (WB) (Western Blot analysis of DDX41 expression in transfected 293T cell line by DDX41 monoclonal antibody. Lane 1: DDX41 transfected lysate (7kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to DDX41 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged DDX41 is ~0.3ng/ml as a capture antibody.)
Western Blot (WB) (DDX6 Antibody (Center) western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).This demonstrates the DDX6 antibody detected the DDX6 protein (arrow).)
Immunohistochemistry (IHC) (DDX6 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Testing Data (24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x106) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios. Data courtesy of Dr. Samantha Furr, University of North Carolina at Charlotte.)
Testing Data (24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x106) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios. Data courtesy of Dr. Samantha Furr, University of North Carolina at Charlotte.)
Western Blot (WB) (EIF4A3 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the EIF4A3 antibody detected the EIF4A3 protein (arrow).)
Western Blot (WB) (Western blot analysis of DDX5 antibody (C-term) in T47D cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB) (Western blot analysis of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).)
Western Blot (WB) (Western blot analysis of DDX3 Antibody (C-term) in Hela cell line lysates (35ug/lane). DDX3 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Testing Data (24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x106) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios. Data courtesy of Dr. Samantha Furr, University of North Carolina at Charlotte.)
Western Blot (WB) (Western Blot detection against Immunogen (132.46kD).)
Western Blot (WB) (DDX11 monoclonal antibody. Western Blot analysis of DDX11 expression in human liver.)
Western Blot (WB) (DDX11 monoclonal antibody Western Blot analysis of DDX11 expression in HeLa.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to DDX11 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
Testing Data (Detection limit for recombinant GST tagged DDX11 is 3ng/ml as a capture antibody)
Western Blot (WB) (DDX11 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the DDX11 antibody detected the DDX11 protein (arrow).)
Flow Cytometry (FC/FACS) (DDX11 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using 210442 at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectal cancer using DDX58 antibody at dilution of 1:200 (200x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cell using DDX58 antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western blot analysis of MBS628323 to detect PIAS1 in HL-60 tissue lysate.)
Western Blot (WB) (Western blot analysis of PIAS1 (arrow) using MBS628323 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS628323 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.)
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