Protein Kinase D2 (PKD2) Recombinant Protein | PKD2 recombinant protein

Recombinant Protein Kinase D2 (PKD2)

Cat. Num. AAA2018552

• Gene Names: PRKD2; PKD2; HSPC187; nPKC-D2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Kinase D2 (PKD2); Recombinant Protein Kinase D2 (PKD2); PKD2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EKVFVVMEKL HGDMLEMILS SEKGRLPERL TKFLITQILV ALRHLHFKNI VHCDLKPENV LLASADPFPQ VKLCDFGFAR IIGEKSFRRS VVGTPAYLAP EVLLNQGYNR SLDMWSVGVI MYVSLSGTFP FNEDEDINDQ IQNAAFMYPA SPWSHISAGA IDLINNLLQV KMRKRYSVDK SLSHPWLQEY QTWLDLRELEGKMGERYITH ES
• Sequence Length: 878

• Applicable Applications for PKD2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Glu621~Ser832 (Accession # Q9BZL6) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm. Cell membrane. Nucleus. Golgi apparatus, trans-Golgi network
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 120659782
• NCBI GeneID: 25865
• NCBI Accession #: NP_001073349.1
• NCBI GenBank Nucleotide #: NM_001079880.1
• UniProt Accession #: Q9BZL6; Q8TB08; Q9P0T6; Q9Y3X8
• Molecular Weight: 28.2kDa
• NCBI Official Full Name: serine/threonine-protein kinase D2 isoform A
• NCBI Official Synonym Full Names: protein kinase D2
• NCBI Official Symbol: PRKD2
• NCBI Official Synonym Symbols: PKD2; HSPC187; nPKC-D2
• NCBI Protein Information: serine/threonine-protein kinase D2
• UniProt Protein Name: Serine/threonine-protein kinase D2
• Protein Family: Polycystin
• UniProt Gene Name: PRKD2
• UniProt Synonym Gene Names: PKD2
• UniProt Entry Name: KPCD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PRKD2: a calcium-independent, phospholipid-dependent CAMK kinase of the PKD family. Activated by diacylglycerol and phorbol esters. may play a role in carcinoid tumor progression.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Motility/polarity/chemotaxis; Kinase, protein; EC 2.7.11.13; CAMK group; PKD family

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: cell death; positive regulation of cell adhesion; protein amino acid autophosphorylation; positive regulation of blood vessel endothelial cell migration; activation of CREB transcription factor; positive regulation of fibroblast growth factor receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; peptidyl-serine phosphorylation; positive regulation of angiogenesis; positive regulation of T cell receptor signaling pathway; positive regulation of interleukin-2 production; positive regulation of transcription factor activity; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; cell adhesion; vascular endothelial growth factor receptor signaling pathway