Rabbit p68 RNA Helicase Polyclonal Antibody | anti-p68RNA antibody

p68 RNA Helicase (Phospho-Y593) Polyclonal Antibody

Cat. Num. AAA3005054

• Gene Names: DDX5; p68; HLR1; G17P1; HUMP68
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: p68 RNA Helicase; Polyclonal Antibody; p68 RNA Helicase (Phospho-Y593) Polyclonal Antibody; ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5; DEAD (Asp Glu Ala Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase; 68kD); DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase 68kDa); DEAD/H box-5 (RNA helicase 68kD); DKFZp686J01190; G17P1; HELR; HLR 1; HLR1; HUMP68; P68; p68 RNA he; anti-p68RNA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p68 RNA Helicase (phospho-Y593) polyclonal antibody detects endogenous levels of p68 RNA Helicase protein only when phosphorylated at Tyr593.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 614

• Applicable Applications for anti-p68RNA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide derived from human p68 RNA Helicase around the phosphorylation site of Y593.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p68 RNA Helicase (phospho-Y593) polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:CT-26 whole cell lysate (40ug) Lane5:C6 whole cell lysate (40ug))

Related Product Information for anti-p68RNA antibody

p68 RNA Helicase is a nuclear protein that exhibits RNA-dependent ATPase activity. Phosphorylation by protein kinase C inhibits p68 RNA Helicase activity. p68 RNA Helicase appears to play a role in organ differentiation during development. Furthermore, p68 RNA Helicase is expressed in early neural development and in various mesodermal tissues in a number of different chordate embryos. At the cellular level, the expression levels of p68 RNA Helicase increase in serum-induced quiescent cell lines. p68 RNA Helicase may function as a coactivator for estrogen receptor alpha. Additionally, p68 RNA Helicase associates with transcriptional coactivators CBP and p300. p68 RNA Helicase localizes to the nucleus under normal conditions. During late telophase, p68 RNA Helicase and fibrillarin co-localize to nascent nucleoli. p68 RNA Helicase may function as a heterodimer with p72 RNA Helicase.

NCBI and Uniprot Product Information

• NCBI GI #: 4758138
• NCBI GeneID: 1655
• NCBI Accession #: NP_004387.1
• NCBI GenBank Nucleotide #: NP_004387.1
• UniProt Accession #: P17844
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX5 isoform a
• NCBI Official Synonym Full Names: DEAD-box helicase 5
• NCBI Official Symbol: DDX5
• NCBI Official Synonym Symbols: p68; HLR1; G17P1; HUMP68
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX5
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX5
• UniProt Gene Name: DDX5
• UniProt Synonym Gene Names: G17P1; HELR; HLR1
• UniProt Entry Name: DDX5_HUMAN

NCBI Description

This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]

Uniprot Description

DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: RNA splicing; Spliceosome; Nuclear receptor co-regulator; Helicase; EC 3.6.4.13; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; membrane; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; RNA helicase activity; ATP-dependent RNA helicase activity; ATP binding

Biological Process: circadian rhythm; nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of osteoblast differentiation; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome