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Western Blot (WB)
(Western Blot detection against Immunogen (36.89kD).)
Mouse anti-Human, Rat DDX56 Monoclonal Antibody | anti-DDX56 antibody
DDX56 (Probable ATP-dependent RNA Helicase DDX56, ATP-dependent 61kD Nucleolar RNA Helicase, DEAD Box Protein 21, DEAD Box Protein 56, DDX21, NOH61) (Biotin)
Reactivity
Human, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DDX56; Monoclonal Antibody; DDX56 (Probable ATP-dependent RNA Helicase DDX56; ATP-dependent 61kD Nucleolar RNA Helicase; DEAD Box Protein 21; DEAD Box Protein 56; DDX21; NOH61) (Biotin); anti-DDX56 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4C5
Specificity
Recognizes human DDX56. Species Crossreactivity: rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-DDX56 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 10ug/ml
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa450-548 from human DDX56 (NP_061955) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
IKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKFRPTAKPS
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (36.89kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.89kD).)
Western Blot (WB)
(Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data
(Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
Western Blot (WB)
(DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Western Blot (WB)
(DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Western Blot (WB)
(DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
Western Blot (WB)
(DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
Product Categories/Family for anti-DDX56 antibody
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Protein Family
Similar Products
Product Notes
The DDX56 (Catalog #AAA6141507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DDX56 (Probable ATP-dependent RNA Helicase DDX56, ATP-dependent 61kD Nucleolar RNA Helicase, DEAD Box Protein 21, DEAD Box Protein 56, DDX21, NOH61) (Biotin) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DDX56 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 10ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DDX56 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX56, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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