Western Blot (WB) (Protein A antibody was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of)
Western Blot (Western Blot analysis using GFP antibody1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 ug (2) and 10 ug (3) of cell lysates used. )
Western Blot (WB) ()
Immunofluorescence (Immunofluorescent staining of GFP antibodySf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification). Sf-1:Cre mice were crossed to the Z/EG reporter line. Brains were harvested following cardiac perfusion with 4% PFA/PBS with ON fixation, and subsequently transferred to a 30% sucrose solution. Brains were then frozen in OCT freezing medium and cryostat sectioned at 40 microns. GFP antibody was used at 1:500 dilution in free floating imunnohistochemistry to detect eGFP. Fluorchrome conj ugated anti-Goat IgG secondary antibody was used for detection at 1:500. Sections were counterstained with DAPI. )
Western Blot (Western Blot analysis using Mammaglobin 1 antibodyWestern Blot showing Mammaglobin-1 antibody used against full-length GST- Mammaglobin-1 (1) and full-length MBP- Mammaglobin-1 (aa1-193) recombinant protein (2).)
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