Western Blot
(Western Blot analysis using GFP antibody1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 ug (2) and 10 ug (3) of cell lysates used. )
Mouse GFP Monoclonal Antibody | anti-GFP antibody
GFP antibody
Applications
Dot Blot, ELISA, Immunoprecipitation, Western Blot
Purity
GFP antibody was purified by Protein A affinity chromatography.
Synonyms
GFP; Monoclonal Antibody; GFP antibody; Monoclonal GFP; Anti-GFP; Green Fluorescent Protein; GFP tag; anti-GFP antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
GF28R
Specificity
GFP
Purity/Purification
GFP antibody was purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3
Concentration
1 mg/ml (varies by lot)
Sequence Length
238
Applicable Applications for anti-GFP antibody
Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: 1:1000-3000
Biological Significance
Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry.
Immunogen
GFP antibody was raised in mouse using GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates as the immunogen.
Preparation and Storage
Store at 4 degree C for several days to weeks. Add 0.1% NaN3 and store at -20 degree C for longer periods. Avoid repeated freeze/thaw cycles.
Western Blot
(Western Blot analysis using GFP antibody1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 ug (2) and 10 ug (3) of cell lysates used. )
Western Blot
(Western Blot analysis using GFP antibody1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 ug (2) and 10 ug (3) of cell lysates used. )
Related Product Information for anti-GFP antibody
Mouse monoclonal GFP antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GFP (Catalog #AAA830408) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). WB: 1:1000-3000. Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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