Western Blot (WB)
(Protein A antibody was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of)
Goat Protein A Polyclonal Antibody | anti-faeC antibody
Protein A antibody (biotin)
Reactivity
Staphylococcus aureus protein A
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Protein A antibody (biotin) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Synonyms
Protein A; Polyclonal Antibody; Protein A antibody (biotin); Polyclonal Protein A; Anti-Protein A; anti-faeC antibody
Host
Goat
Reactivity
Staphylococcus aureus protein A
Clonality
Polyclonal
Specificity
Staphylococcus aureus protein A
Purity/Purification
Protein A antibody (biotin) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form/Format
IgG fraction supplied as a liquid in 20mM Potassium Phosphate and 150mM NaCl, pH 7.2, with 10mg/ml BSA (IgG and Protease free) as a stabilizer, and 0.01% NaN3.
Sequence Length
181
Applicable Applications for anti-faeC antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:20,000-1:100,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
Biological Significance
Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It binds proteins from many of mammalian species, most notably IgGs. It binds with the Fc region of immunoglobulins through interaction with the heavy chain.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Tag/Conjugate
Biotin
Immunogen
Protein A antibody (biotin) was raised in goat using Protein A [Staphylococcus aureus] as the immunogen.
Preparation and Storage
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Western Blot (WB)
(Protein A antibody was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of)
Western Blot (WB)
(Protein A antibody was used to detect Protein A under reducing and non-reducing conditions. Reduced samples of purified Protein A contained 4% BME and were boiled for 5 minutes. Samples of)
Related Product Information for anti-faeC antibody
Goat polyclonal Protein A antibody (biotin) conjugated
Product Categories/Family for anti-faeC antibody
NCBI and Uniprot Product Information
Uniprot Description
Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.
Similar Products
Product Notes
The Protein A faec (Catalog #AAA536128) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Protein An antibody (biotin) reacts with Staphylococcus aureus protein An and may cross-react with other species as described in the data sheet. AAA Biotech's Protein A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:20,000-1:100,000 IHC: 1:1,000-1:5,000 WB: 1:2,000-1:10,000. Researchers should empirically determine the suitability of the Protein A faec for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Protein A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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