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Application
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402 results for " Lipid Signaling" - showing 200-250
Western Blot (WB)
(Western blot analysis of CYP2E1 expression in HeLa cell lysate (MBS179408).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 )
CYP2E1, Monoclonal Antibody (Cat# AAA179408)
Full Name
Anti-CYP2E1 Rabbit Monoclonal Antibody
Gene Names
CYP2E1; CPE1; CYP2E; P450-J; P450C2E
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001068)
Full Name
RAC1 Antibody: Biotin
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of LXR alpha expression in Jurkat cell lysate (MBS179951).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1H3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NR1H3 )
LXR alpha, Monoclonal Antibody (Cat# AAA179951)
Full Name
Anti-LXR alpha Rabbit Monoclonal Antibody
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rac1/2/3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate (MBS179055).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAC1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAC1 )
Rac1/2/3, Monoclonal Antibody (Cat# AAA179055)
Full Name
Anti-Rac1/2/3 Rabbit Monoclonal Antibody
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Host: RabbitTarget Name: PPAP2CSample Tissue: Human Hela Whole Cell lysatesAntibody Dilution: 1ug/ml)
PLPP2, Polyclonal Antibody (Cat# AAA3222216)
Full Name
PLPP2 Antibody - C-terminal region
Gene Names
PLPP2; LPP2; PAP-2c; PAP2-g; PPAP2C
Reactivity
Human
Applications
WB
Purity
Affinity purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-SHIP (Y1020) expression in (1) Raji cell lysate; (2) Raji cell treated with pervanadate lysate (MBS1750279).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5D monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for INPP5D )
SHIP, Monoclonal Antibody (Cat# AAA1750279)
Full Name
Anti-Phospho-SHIP (Y1020) Rabbit Monoclonal Antibody
Gene Names
INPP5D; SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship
Reactivity
Human
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.)
NRAS, Polyclonal Antibody (Cat# AAA8001770)
Full Name
NRAS Antibody: Biotin
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001057)
Full Name
RAC1 Antibody
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.)
GABARAPL3, Polyclonal Antibody (Cat# AAA809510)
Full Name
GABARAPL3 Antibody: ATTO 488
Gene Names
GABARAPL3; ATG8D
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.)
GABARAPL3, Polyclonal Antibody (Cat# AAA809524)
Full Name
GABARAPL3 Antibody: RPE
Gene Names
GABARAPL3; ATG8D
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Cytochrome C expression in HeLa cell lysate (MBS179971).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYCS )
Cytochrome C, Monoclonal Antibody (Cat# AAA179971)
Full Name
Anti-Cytochrome C Rabbit Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of TRAF6 expression in Jurkat cell lysate (MBS179172).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TRAF6 )
TRAF6, Monoclonal Antibody (Cat# AAA179172)
Full Name
Anti-TRAF6 Rabbit Monoclonal Antibody
Gene Names
TRAF6; RNF85; MGC:3310
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Cytochrome C expression in Human kidney lysate (MBS179972).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYCS )
Cytochrome C, Monoclonal Antibody (Cat# AAA179972)
Full Name
Anti-Cytochrome C Rabbit Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.)
PIK3R4, Polyclonal Antibody (Cat# AAA809854)
Full Name
PIK3R4 Antibody: ATTO 594
Gene Names
PIK3R4; p150; VPS15
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.)
NRAS, Polyclonal Antibody (Cat# AAA8001760)
Full Name
NRAS Antibody: ATTO 390
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001426)
Full Name
KRAS Antibody: Alkaline Phosphatase
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001428)
Full Name
KRAS Antibody: Biotin
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PLCG1 expression in Jurkat cell lysate (MBS179411).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCG1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLCG1 )
PLCG1/Plc Gamma 1, Monoclonal Antibody (Cat# AAA179411)
Full Name
Anti-PLCG1/Plc Gamma 1 Rabbit Monoclonal Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.)
GABARAPL3, Polyclonal Antibody (Cat# AAA809517)
Full Name
GABARAPL3 Antibody: Alkaline Phosphatase
Gene Names
GABARAPL3; ATG8D
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001059)
Full Name
RAC1 Antibody: ATTO 488
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~21.3 kDa HRAS protein using Rabbit Anti-HRAS Polyclonal Antibody (SPC-776). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HRAS Polyclonal Antibody (SPC-776) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.3 kDa. Other Band(s): 25 kDa.)
HRAS, Polyclonal Antibody (Cat# AAA8001399)
Full Name
HRAS Antibody
Gene Names
HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001066)
Full Name
RAC1 Antibody: Alkaline Phosphatase
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-Rac1/Cdc42 (Ser71) expression in (1) A431 cell lysate treated with LP; (2) A431 cell lysate (MBS1750211).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC42 )
Rac1/Cdc42, Monoclonal Antibody (Cat# AAA1750211)
Full Name
Anti-Phospho-Rac1/Cdc42 (Ser71) Rabbit Monoclonal Antibody
Gene Names
CDC42; TKS; G25K; CDC42Hs
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.)
GABARAPL3, Polyclonal Antibody (Cat# AAA809512)
Full Name
GABARAPL3 Antibody: ATTO 594
Gene Names
GABARAPL3; ATG8D
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~21.3 kDa HRAS protein using Rabbit Anti-HRAS Polyclonal Antibody (SPC-776). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HRAS Polyclonal Antibody (SPC-776) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.3 kDa. Other Band(s): 25 kDa.)
HRAS, Polyclonal Antibody (Cat# AAA8001401)
Full Name
HRAS Antibody: ATTO 488
Gene Names
HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PTGER4 using anti- PTGER4 antibody (MBS1750748).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: SW620 whole Cell lysates,Lane 2: MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PTGER4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTGER4 at approximately 53KD. The expected band size for PTGER4 is at 53KD. )
PTGER4, Polyclonal Antibody (Cat# AAA1750748)
Full Name
Anti-PTGER4 Picoband Antibody
Gene Names
PTGER4; EP4; EP4R
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001072)
Full Name
RAC1 Antibody: PerCP
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
STARD13, Polyclonal Antibody (Cat# AAA7109687)
Full Name
STARD13 Antibody, Biotin conjugated
Gene Names
STARD13; DLC2; GT650; ARHGAP37; LINC00464
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.)
PIK3R4, Polyclonal Antibody (Cat# AAA809862)
Full Name
PIK3R4 Antibody: FITC
Gene Names
PIK3R4; p150; VPS15
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of ~21.6 kDa KRAS protein using Rabbit Anti-KRAS Polyclonal Antibody (SPC-777). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-KRAS Polyclonal Antibody (SPC-777) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.6 kDa. Other Band(s): ~29 kDa (PTM).)
KRAS, Polyclonal Antibody (Cat# AAA8001430)
Full Name
KRAS Antibody: HRP
Gene Names
KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.)
GABARAPL3, Polyclonal Antibody (Cat# AAA809508)
Full Name
GABARAPL3 Antibody
Gene Names
GABARAPL3; ATG8D
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.)
GABARAPL3, Polyclonal Antibody (Cat# AAA809509)
Full Name
GABARAPL3 Antibody: ATTO 390
Gene Names
GABARAPL3; ATG8D
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CaMKII alpha expression in SH-SY5Y cell lysate (MBS179946).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMK2A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAMK2A )
CaMKII alpha, Monoclonal Antibody (Cat# AAA179946)
Full Name
Anti-CaMKII alpha Rabbit Monoclonal Antibody
Gene Names
CAMK2A; CAMKA; MRD53
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001061)
Full Name
RAC1 Antibody: ATTO 594
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Liver showing detection of ~47.2 kDa PTEN protein using Rabbit Anti-PTEN Polyclonal Antibody (SPC-766). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PTEN Polyclonal Antibody (SPC-766) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~47.2 kDa. Other Band(s): 67 kDa.)
PTEN, Polyclonal Antibody (Cat# AAA8001232)
Full Name
PTEN Antibody: HRP
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Liver showing detection of ~47.2 kDa PTEN protein using Rabbit Anti-PTEN Polyclonal Antibody (SPC-766). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PTEN Polyclonal Antibody (SPC-766) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~47.2 kDa. Other Band(s): 67 kDa.)
PTEN, Polyclonal Antibody (Cat# AAA8001219)
Full Name
PTEN Antibody
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CDC42 expression in (1) Jurkat cell lysate; (2) Mouse spleen lysate (MBS179121).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC42 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC42 )
CDC42, Monoclonal Antibody (Cat# AAA179121)
Full Name
Anti-CDC42 Rabbit Monoclonal Antibody
Gene Names
CDC42; TKS; G25K; CDC42Hs
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence staining of MCF-7 cells with MBS7109684 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
STARD13, Polyclonal Antibody (Cat# AAA7109684)
Full Name
STARD13 Antibody
Gene Names
STARD13; DLC2; GT650; ARHGAP37; LINC00464
Reactivity
Human
Applications
EIA, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.)
NRAS, Polyclonal Antibody (Cat# AAA8001771)
Full Name
NRAS Antibody: FITC
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (SPC-660) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.)
PIK3R4, Polyclonal Antibody (Cat# AAA809866)
Full Name
PIK3R4 Antibody: RPE
Gene Names
PIK3R4; p150; VPS15
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Liver cell lysates showing detection of 14 kDa LC3A protein using Rabbit Anti-LC3A Polyclonal Antibody (SPC-613). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody (SPC-613) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
LC3A, Polyclonal Antibody (Cat# AAA809454)
Full Name
LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Liver cell lysates showing detection of 14 kDa LC3A protein using Rabbit Anti-LC3A Polyclonal Antibody (SPC-613). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody (SPC-613) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
LC3A, Polyclonal Antibody (Cat# AAA809458)
Full Name
LC3A Antibody: ATTO 594
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human; Mouse; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~21 kDa RAC1 protein using Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757). Lane 1: Molecular Weight Ladder (MW). Lane 2: HEK293T . Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAC1 Polyclonal Antibody (SPC-757) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.)
RAC1, Polyclonal Antibody (Cat# AAA8001069)
Full Name
RAC1 Antibody: FITC
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAPL3 Antibody (D) Composite.)
GABARAPL3, Polyclonal Antibody (Cat# AAA809523)
Full Name
GABARAPL3 Antibody: PerCP
Gene Names
GABARAPL3; ATG8D
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of ~21.3 kDa HRAS protein using Rabbit Anti-HRAS Polyclonal Antibody (SPC-776). Lane 1: Molecular Weight Ladder (MW). Lane 2: A549. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HRAS Polyclonal Antibody (SPC-776) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21.3 kDa. Other Band(s): 25 kDa.)
HRAS, Polyclonal Antibody (Cat# AAA8001414)
Full Name
HRAS Antibody: PerCP
Gene Names
HRAS; CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Liver showing detection of ~47.2 kDa PTEN protein using Rabbit Anti-PTEN Polyclonal Antibody (SPC-766). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PTEN Polyclonal Antibody (SPC-766) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~47.2 kDa. Other Band(s): 67 kDa.)
PTEN, Polyclonal Antibody (Cat# AAA8001221)
Full Name
PTEN Antibody: ATTO 488
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Liver showing detection of ~47.2 kDa PTEN protein using Rabbit Anti-PTEN Polyclonal Antibody (SPC-766). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PTEN Polyclonal Antibody (SPC-766) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~47.2 kDa. Other Band(s): 67 kDa.)
PTEN, Polyclonal Antibody (Cat# AAA8001235)
Full Name
PTEN Antibody: RPE
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PI 3 Kinase p85 beta expression in HeLa cell lysate (MBS179955).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3R2 )
PI 3 Kinase p85 beta, Monoclonal Antibody (Cat# AAA179955)
Full Name
Anti-PI 3 Kinase p85 beta Rabbit Monoclonal Antibody
Gene Names
PIK3R2; p85; MPPH; P85B; MPPH1; p85-BETA
Reactivity
Human, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunoblot (IB)
(A 1:1000 dilution was sufficient for detection of ELOVL3 in 10 ug of Rat brain by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.)
ELOVL3, Monoclonal Antibody (Cat# AAA8001953)
Full Name
ELOVL3 Antibody: Biotin
Gene Names
ELOVL3; CIG30; CIG-30
Reactivity
Human, Rat
Applications
WB, IHC, EIA
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.)
NRAS, Polyclonal Antibody (Cat# AAA8001774)
Full Name
NRAS Antibody: PerCP
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
