Rabbit LC3A Polyclonal Antibody | anti-LC3A antibody

LC3A Antibody: ATTO 594

Cat. Num. AAA809458

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Reactivity: Human; Mouse; Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Peptide Affinity Purified
• Synonyms: LC3A; Polyclonal Antibody; LC3A Antibody: ATTO 594; Rabbit Anti-Human LC3A Polyclonal; MAP1 light chain 3 like protein 1 Antibody; Map1lc3a Antibody; MAP1BLC3 Antibody; Autophagy-related ubiquitin-like modifier LC3 A Antibody; MAP1LC3A Antibody; LC3A Antibody; Microtubule-associated proteins 1A and 1B; light chain 3 Antibody; MAP1ALC3 Antibody; MAP1A/1B light chain 3 A Antibody; MAP1A/MAP1B light chain 3 A Antibody; Microtubule-associated protein 1 light chain 3 alpha Antibody; LC3 Antibody; Microtubule-associated proteins 1A/1B light chain 3A Antibody; Autophagy-related protein LC3 A Antibody; MAP1A/MAP1B LC3 A Antibody; ATG8E Antibody; MLP3A_HUMAN Antibody; MAP1 light chain 3-like protein 1 Antibody; Microtubule associated proteins 1A/1B light chain 3 Antibody; anti-LC3A antibody

• Host: Rabbit
• Reactivity: Human; Mouse; Rat
• Clonality: Polyclonal
• Specificity: Detects ~14 kDa. Band at 50 kDa is LC3A complex.
• Purity/Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 121

• Applicable Applications for anti-LC3A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Target: LC3A
• Immunogen: Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12)
• Immunogen Species: Human
• Cellular Localization: Cytoplasm; Cytoskeleton; Endomembrane System; Lipid-Anchor; Cytoplasmic Vesicle; Autophagosome Membrane; Lipid-Anchor; Cytoplasmic Vesicle; Autophagosome
• Conjugate: ATTO 594
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Tissue Specificity: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Rat Liver cell lysates showing detection of 14 kDa LC3A protein using Rabbit Anti-LC3A Polyclonal Antibody (SPC-613). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody (SPC-613) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)

Related Product Information for anti-LC3A antibody

Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.

Product Categories/Family for anti-LC3A antibody

Antibodies; Cancer; Autophagy; Cell Signaling; Neuroscience; Cardiovascular System; Heart

References

1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.

NCBI and Uniprot Product Information

• NCBI GI #: 14210522
• NCBI GeneID: 84557
• NCBI Accession #: NP_115903.1
• NCBI GenBank Nucleotide #: NM_032514.3
• UniProt Accession #: Q9H492; Q9BXW5; E1P5P4; E1P5P5
• Molecular Weight: 14,493 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3A isoform a
• NCBI Official Synonym Full Names: microtubule associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600).