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Western Blot (WB) (Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.)

Rabbit anti-Rat NRAS Polyclonal Antibody | anti-NRAS antibody

NRAS Antibody: FITC

Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Rat
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
NRAS; Polyclonal Antibody; NRAS Antibody: FITC; Rabbit Anti-Human NRAS Polyclonal; Transforming protein N-Ras Antibody; GTPase NRas Antibody; NRAS Antibody; HRAS1 Antibody; anti-NRAS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
Detects 25 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
189
Applicable Applications for anti-NRAS antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
NRAS
Immunogen
Synthetic peptide from the C-terminal of Human GTPase NRas (aa. 85-185)
Immunogen Species
Human
Cellular Localization
Cell Membrane; Lipid-Anchor; Cytoplasmic Side; Golgi Apparatus Membrane; Lipid-Anchor
Conjugate
FITC
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.)

Western Blot (WB) (Western blot analysis of Rat Kidney showing detection of 21.2 kDa NRAS protein using Rabbit Anti-NRAS Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney . Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-NRAS Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 21.2 kDa. Other Band(s): 25 kDa.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,229 Da
NCBI Official Full Name
GTPase NRas
NCBI Official Synonym Full Names
NRAS proto-oncogene, GTPase
NCBI Official Symbol
NRAS
NCBI Official Synonym Symbols
NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
NCBI Protein Information
GTPase NRas
UniProt Protein Name
GTPase NRas
Protein Family
UniProt Gene Name
NRAS
UniProt Synonym Gene Names
HRAS1

NCBI Description

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Uniprot Description

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Research Articles on NRAS

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Product Notes

The NRAS nras (Catalog #AAA8001771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NRAS Antibody: FITC reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NRAS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the NRAS nras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NRAS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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