Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
359 results for "Metabolism Cardiovascular System" - showing 150-200
Immunohistochemistry (IHC)
(IHC image of MBS7110291 diluted at 1:200 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
KLB, Polyclonal Antibody (Cat# AAA7110291)
Full Name
KLB Antibody
Gene Names
KLB; BKL
Reactivity
Human
Applications
EIA, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.)
ATG3, Polyclonal Antibody (Cat# AAA809949)
Full Name
ATG3 Antibody: Alkaline Phosphatase
Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney, using AMPK alpha 1 Antibody(MBS179515)PRKAA1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRKAA1 Antibody (MBS179515)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
AMPK alpha 1, Monoclonal Antibody (Cat# AAA179515)
Full Name
Anti-AMPK alpha 1 Rabbit Monoclonal Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of CAV1 in A549 cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Caveolin-1, Monoclonal Antibody (Cat# AAA200503)
Full Name
Caveolin-1
Gene Names
CAV1; CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085
Reactivity
Human
Applications
EIA, WB, ICC, IF
Purity
By protein-A affinity chromatography
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ATG5 expression in Raji cell lysate (MBS179216).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATG5 )
ATG5/Apg5, Monoclonal Antibody (Cat# AAA179216)
Full Name
Anti-ATG5/Apg5 Rabbit Monoclonal Antibody
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(IHC image of MBS7108627 diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
SLC25A37, Polyclonal Antibody (Cat# AAA7108627)
Full Name
SLC25A37 Antibody
Gene Names
SLC25A37; MSC; MFRN; MSCP; HT015; MFRN1; PRO1278; PRO1584; PRO2217
Reactivity
Human
Applications
EIA, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.)
AMBRA1, Polyclonal Antibody (Cat# AAA809746)
Full Name
AMBRA1 Antibody: ATTO 594
Gene Names
AMBRA1; DCAF3; WDR94
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.)
ATG3, Polyclonal Antibody (Cat# AAA809953)
Full Name
ATG3 Antibody: HRP
Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PCSK9 expression in HepG2 cell lysate (MBS179089).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PCSK9 )
PCSK9/Proprotein Convertase 9, Monoclonal Antibody (Cat# AAA179089)
Full Name
Anti-PCSK9/Proprotein Convertase 9 Rabbit Monoclonal Antibody
Gene Names
PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3
Reactivity
Human
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody (SPC-644) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.)
AMBRA1, Polyclonal Antibody (Cat# AAA809743)
Full Name
AMBRA1 Antibody: ATTO 390
Gene Names
AMBRA1; DCAF3; WDR94
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809764)
Full Name
ATG9B Antibody: ATTO 594
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809771)
Full Name
ATG9B Antibody: Biotin
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western BlotPositive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysateAll lanes: DHCR7 antibody at 2.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 55 KDaObserved band size: 55 KDa)
DHCR7, Polyclonal Antibody (Cat# AAA7109827)
Full Name
DHCR7 Antibody
Gene Names
DHCR7; SLOS
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (MBS1750703).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD. )
Cardiac FABP, Polyclonal Antibody (Cat# AAA1750703)
Full Name
Anti-Cardiac FABP Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate (MBS1750246).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAA1 )
AMPK alpha 1, Monoclonal Antibody (Cat# AAA1750246)
Full Name
Anti-Phospho-AMPK alpha 1 (S496) Rabbit Monoclonal Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PRDX6 expression in HeLa cell lysate (MBS179751).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRDX6 )
PRDX6/Peroxiredoxin 6, Monoclonal Antibody (Cat# AAA179751)
Full Name
Anti-PRDX6/Peroxiredoxin 6 Rabbit Monoclonal Antibody
Gene Names
PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SERCA2 expression in HeLa cell lysate (MBS179578).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP2A2 )
SERCA2, Monoclonal Antibody (Cat# AAA179578)
Full Name
Anti-SERCA2 Rabbit Monoclonal Antibody
Gene Names
ATP2A2; DD; DAR; ATP2B; SERCA2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(IHC image of MBS7107215 diluted at 1:200 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
PON3, Polyclonal Antibody (Cat# AAA7107215)
Full Name
PON3 Antibody
Reactivity
Human
Applications
EIA, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunocytochemistry (ICC)/Immunofluorescence (IF)
(ICC/IF analysis of Hexokinase in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Hexokinase, Monoclonal Antibody (Cat# AAA201212)
Full Name
Hexokinase
Gene Names
HK1; HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase
Reactivity
Human
Applications
EIA, WB, ICC, IF
Purity
By protein-A affinity chromatography
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809761)
Full Name
ATG9B Antibody: ATTO 390
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809773)
Full Name
ATG9B Antibody: HRP
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Glycogen synthase expression in HeLa cell lysate (MBS179969).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GYS1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GYS1 )
Glycogen synthase, Monoclonal Antibody (Cat# AAA179969)
Full Name
Anti-Glycogen synthase Rabbit Monoclonal Antibody
Gene Names
GYS1; GSY; GYS
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CEBP alpha expression in U937 cell lysate (MBS179300).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEBPA )
CEBP alpha, Monoclonal Antibody (Cat# AAA179300)
Full Name
Anti-CEBP alpha Rabbit Monoclonal Antibody
Gene Names
CEBPA; CEBP; C/EBP-alpha
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.)
ATG3, Polyclonal Antibody (Cat# AAA809942)
Full Name
ATG3 Antibody: ATTO 488
Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.)
TCF7L2, Polyclonal Antibody (Cat# AAA8000429)
Full Name
TCF7L2 Antibody: ATTO 488
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.)
VMP1, Polyclonal Antibody (Cat# AAA8000006)
Full Name
VMP1 Antibody: Biotin
Gene Names
VMP1; EPG3; TANGO5; TMEM49
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG3 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG3 Antibody. (D) Composite.)
ATG3, Polyclonal Antibody (Cat# AAA809951)
Full Name
ATG3 Antibody: Biotin
Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of TCF7L2 expression in Jurkat cell lysate (MBS179320).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF7L2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCF7L2 )
TCF7L2, Monoclonal Antibody (Cat# AAA179320)
Full Name
Anti-TCF7L2 Rabbit Monoclonal Antibody
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(IHC image of MBS7107819 diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
CLPS, Polyclonal Antibody (Cat# AAA7107819)
Full Name
CLPS Antibody
Reactivity
Human
Applications
EIA, IHC
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of G6PD in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
G6PD, Monoclonal Antibody (Cat# AAA200495)
Full Name
G6PD
Gene Names
G6PD; G6PD1
Reactivity
Human
Applications
EIA, WB, ICC, IF
Purity
Protein-A Affinity Chromatography
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.)
VMP1, Polyclonal Antibody (Cat# AAA8000008)
Full Name
VMP1 Antibody: HRP
Gene Names
VMP1; EPG3; TANGO5; TMEM49
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809769)
Full Name
ATG9B Antibody: Alkaline Phosphatase
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
ATG9B, Polyclonal Antibody (Cat# AAA809772)
Full Name
ATG9B Antibody: FITC
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Immunohistochemistry (IHC)
(IHC image of MBS7109084 diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
VLDLR, Polyclonal Antibody (Cat# AAA7109084)
Full Name
VLDLR Antibody
Gene Names
VLDLR; CAMRQ1; CARMQ1; CHRMQ1; VLDL-R; VLDLRCH
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG10 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG10 Antibody . (D) Composite.)
ATG10, Polyclonal Antibody (Cat# AAA809931)
Full Name
ATG10 Antibody: Alkaline Phosphatase
Gene Names
ATG10; APG10; APG10L; pp12616
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of CYP2E1 expression in HeLa cell lysate (MBS179408).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 )
CYP2E1, Monoclonal Antibody (Cat# AAA179408)
Full Name
Anti-CYP2E1 Rabbit Monoclonal Antibody
Gene Names
CYP2E1; CPE1; CYP2E; P450-J; P450C2E
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.)
TCF7L2, Polyclonal Antibody (Cat# AAA8000436)
Full Name
TCF7L2 Antibody: Alkaline Phosphatase
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of LXR alpha expression in Jurkat cell lysate (MBS179951).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1H3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NR1H3 )
LXR alpha, Monoclonal Antibody (Cat# AAA179951)
Full Name
Anti-LXR alpha Rabbit Monoclonal Antibody
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Leptin using anti-Leptin antibody (MBS1750419).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human Leptin protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Leptin at approximately 16KD. The expected band size for Leptin is at 16KD. )
Leptin, Polyclonal Antibody (Cat# AAA1750419)
Full Name
Anti-Leptin Picoband antibody
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate;(2)HeLa cell lysate (MBS179608).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPAS1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EPAS1 )
HIF-2 alpha, Monoclonal Antibody (Cat# AAA179608)
Full Name
Anti-HIF-2 alpha Rabbit Monoclonal Antibody
Gene Names
EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(IHC image of MBS7106517 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
ACOT1, Polyclonal Antibody (Cat# AAA7106517)
Full Name
ACOT1 Antibody
Gene Names
ACOT1; ACH2; CTE-1; LACH2
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.)
TCF7L2, Polyclonal Antibody (Cat# AAA8000427)
Full Name
TCF7L2 Antibody
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Rat kidney lysate showing detection of ~67.9 kDa TCF7L2 protein using Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat kidney lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7L2 Polyclonal Antibody (SPC-722) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~67.9 kDa. Other Band(s): ~55 kDa isoform, ~75 kDa due to post translational modification.)
TCF7L2, Polyclonal Antibody (Cat# AAA8000431)
Full Name
TCF7L2 Antibody: ATTO 594
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Chemical Structure
L-Arginine, Inhibitor (Cat# AAA5750305)
Full Name
L-Arginine
Purity
98.00%
Pricing
$160/5 mg | $195/500 mg | $205/1 g | $645/5x1 g
Western Blot (WB)
(Figure 1. Western blot analysis of Leptin using anti-Leptin antibody (MBS1750418).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant rat Leptin protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Leptin at approximately 16KD. The expected band size for Leptin is at 16KD. )
Leptin, Polyclonal Antibody (Cat# AAA1750418)
Full Name
Anti-Leptin Picoband Antibody
Gene Names
Lep; OB; obese
Reactivity
Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of SOD2 expression in Rat brain lysate (MBS179281).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOD2 )
SOD2/Mnsod, Monoclonal Antibody (Cat# AAA179281)
Full Name
Anti-SOD2/Mnsod Rabbit Monoclonal Antibody
Gene Names
SOD2; IPOB; IPO-B; MNSOD; MVCD6; Mn-SOD
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Cytochrome C expression in HeLa cell lysate (MBS179971).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYCS )
Cytochrome C, Monoclonal Antibody (Cat# AAA179971)
Full Name
Anti-Cytochrome C Rabbit Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Atg4C expression in Jurkat cell lysate (MBS1750116).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4C monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATG4C )
Atg4C, Monoclonal Antibody (Cat# AAA1750116)
Full Name
Anti-Atg4C Rabbit Monoclonal Antibody
Gene Names
ATG4C; APG4C; AUTL1; AUTL3; APG4-C
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of NeuroD1 expression in Y79 cell lysate (MBS179533).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROD1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NEUROD1 )
NeuroD1, Monoclonal Antibody (Cat# AAA179533)
Full Name
Anti-NeuroD1 Rabbit Monoclonal Antibody
Gene Names
NEUROD1; BETA2; BHF-1; MODY6; NEUROD; bHLHa3
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (MBS1750479).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD. )
LOX-1/OLR1, Polyclonal Antibody (Cat# AAA1750479)
Full Name
Anti-LOX-1/OLR1 Picoband Antibody
Gene Names
OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
