Zuotin Related Factor 1 Recombinant Protein | ZRF1 recombinant protein

Recombinant Zuotin Related Factor 1 (ZRF1)

Cat. Num. AAA2033290

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Zuotin Related Factor 1; Recombinant Zuotin Related Factor 1 (ZRF1); MPP11; DNAJC2; MPHOSPH11; ZUO1; DnaJ(Hsp40)Homolog; Subfamily C; Member 2; M-phase phosphoprotein 11; DnaJ homolog subfamily C member 2; N-terminally processed; ZRF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: DNE ADRVKMMEEV EKLCDRLELA SLQGLNEILA SSTREVGKAA LEKQIEEVNE QMRREKEEAD ARMRQASKNA EKSTGGSGSG SKNWSEDDLQ LLIKAVNLFP AGTNSRWEVI ANYMNIHSSS GVKRTAKDVI SKAKSLQKLD PHQKDDINKK AFDKFKKEHG VASQADSAAP SERFEGPCID STPWTTEEQK LLEQALKTYP VNTPERWEKI AEAVPGRTKK DCMRRYKELVEMVKAKKAAQ EQVLNASRAR K
• Sequence Length: 621

• Applicable Applications for ZRF1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 32.3kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asp368~Lys621
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 32481970
• Molecular Weight: 63,504 Da
• NCBI Official Full Name: zuotin related factor 1
• UniProt Protein Name: DnaJ homolog subfamily C member 2
• UniProt Gene Name: Dnajc2
• UniProt Synonym Gene Names: Mida1; Zrf1
• UniProt Entry Name: DNJC2_RAT

Uniprot Description

DNAJC2: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; DNA replication; Chaperone

Cellular Component: cytoplasm; cytosol; nuclear membrane; nucleus

Molecular Function: chromatin binding; DNA binding; histone binding; Hsp70 protein binding; ubiquitin binding

Biological Process: chromatin modification; DNA replication; negative regulation of cell growth; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent