Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of G6PD in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human G6PD Monoclonal Antibody | anti-G6PD antibody
G6PD
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of G6PD in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of G6PD in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Western Blot (WB)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human G6PD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; Lane 1.: MCF7 cell lysate; Lane 2.: HeLa cell lysate; )
Western Blot (WB)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human G6PD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;
Lane 1.: MCF7 cell lysate;
Lane 2.: HeLa cell lysate;
)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.
Protein type: Cell development/differentiation; Other Amino Acids Metabolism - glutathione; Carbohydrate Metabolism - pentose phosphate pathway; EC 1.1.1.49; Oxidoreductase
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: centrosome; internal side of plasma membrane; membrane; intracellular membrane-bound organelle; cytoplasm; microtubule organizing center; nucleus; cytosol
Molecular Function: identical protein binding; glucose-6-phosphate dehydrogenase activity; protein binding; protein homodimerization activity; NADP binding; glucose binding
Biological Process: NADPH regeneration; response to food; pentose-phosphate shunt; erythrocyte maturation; ribose phosphate biosynthetic process; glucose 6-phosphate metabolic process; pentose-phosphate shunt, oxidative branch; glucose metabolic process; cytokine production; pathogenesis; NADP metabolic process; cholesterol biosynthetic process; response to organic cyclic substance; glutathione metabolic process; substantia nigra development; response to ethanol; regulation of neuron apoptosis; carbohydrate metabolic process; lipid metabolic process; pentose biosynthetic process
Disease: Favism, Susceptibility To; Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Malaria, Susceptibility To
Research Articles on G6PD
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Product Notes
The G6PD g6pd (Catalog #AAA200495) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The G6PD reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's G6PD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the G6PD g6pd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G6PD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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