Western Blot (WB) (Mouse Cblb Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Cblb antibody detected the Cblb protein (arrow).)
Western Blot (WB) (Western blot analysis of Rat brain lysate showing detection of ~33.7 kDa Cyclin D1 protein using Rabbit Anti-Cyclin D1 Polyclonal Antibody (SPC-731). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D1 Polyclonal Antibody (SPC-731) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.7 kDa. Other Band(s): ~40 kDa due to post translational modifications.)
Testing Data (Gel: 8%SDS-PAGE Lysates (from left to right): A549 and hela cell Amount of lysate: 40ug per lane Primary antibody: 1/333 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 minutes)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue using at dilution 1/15.)
Dot Blot (DB) (Dot blot analysis of Phospho-bcl-2-S70 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Western Blot (WB) (Western blot analysis of extracts from Jurkat cells,untreated or treated with paclitaxel,using phospho-Bcl2(Ser70)(left) or Bcl2 antibody(right).)