BCL3 sirna

BCL3 siRNA (Mouse)

Cat. Num. AAA8241302

• Gene Names: Bcl3; Bcl-3; AI528691
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BCL3; BCL3 siRNA (Mouse); B-cell lymphoma 3 protein homolog; BCL-3; BCL3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BCL3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 448

• Applicable Applications for BCL3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BCL3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BCL3 sirna

siRNA to inhibit BCL3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 164607184
• NCBI GeneID: 12051
• NCBI Accession #: NP_291079.2
• NCBI GenBank Nucleotide #: NM_033601.3
• UniProt Accession #: Q9Z2F6
• Molecular Weight: 47,216 Da
• NCBI Official Full Name: B-cell lymphoma 3 protein homolog
• NCBI Official Synonym Full Names: B cell leukemia/lymphoma 3
• NCBI Official Symbol: Bcl3
• NCBI Official Synonym Symbols: Bcl-3; AI528691
• NCBI Protein Information: B-cell lymphoma 3 protein homolog
• UniProt Protein Name: B-cell lymphoma 3 protein homolog
• Protein Family: B-cell lymphoma 3 protein
• UniProt Gene Name: Bcl3
• UniProt Synonym Gene Names: BCL-3
• UniProt Entry Name: BCL3_MOUSE

Uniprot Description

Bcl-3: Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.

Protein type: Apoptosis; Transcription regulation; Nuclear receptor co-regulator; Oncoprotein

Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; intercellular bridge; protein complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; transcription factor binding; transcription factor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; positive regulation of translation; DNA damage response, signal transduction by p53 class mediator; positive regulation of transcription, DNA-dependent; follicular dendritic cell differentiation; negative regulation of interleukin-8 biosynthetic process; regulation of apoptosis; humoral immune response mediated by circulating immunoglobulin; regulation of transcription, DNA-dependent; germinal center formation; antimicrobial humoral response; negative regulation of tumor necrosis factor biosynthetic process; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; spleen development; T-helper 1 type immune response; transcription, DNA-dependent; response to virus; defense response to protozoan; response to UV-C; marginal zone B cell differentiation; positive regulation of interferon-gamma production; positive regulation of interleukin-10 biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis