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266 results for " Western Blot Kits" - showing 100-150
Western Blot (WB)
(Western blot analysis of CD31 expression in THP1 cell lysate (MBS179680).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECAM1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PECAM1 )
CD31, Monoclonal Antibody (Cat# AAA179680)
Full Name
Anti-CD31 Rabbit Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho- Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (MBS1750209).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK3 )
Erk1, Monoclonal Antibody (Cat# AAA1750209)
Full Name
Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of IL1RA expression in (1) HeLa cell lysate; (2) 3T3 cell lysate (MBS179404).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RN monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RN )
IL1RA, Monoclonal Antibody (Cat# AAA179404)
Full Name
Anti-IL1RA Rabbit Monoclonal Antibody
Gene Names
IL1RN; DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of STAT6 in expression (1)Daudi cell lysate;(2)HeLa cell lysate (MBS179351).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT6 )
STAT6, Monoclonal Antibody (Cat# AAA179351)
Full Name
Anti-STAT6 Rabbit Monoclonal Antibody
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Human
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of STAT4 expression in Hela cell lysate (MBS179434).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT4 )
STAT4, Monoclonal Antibody (Cat# AAA179434)
Full Name
Anti-STAT4 Rabbit Monoclonal Antibody
Gene Names
STAT4; SLEB11
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of IL1RA using anti-IL1RA antibody (MBS1750456).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RA at approximately 28KD. The expected band size for IL1RA is at 20KD. )
IL1RA, Polyclonal Antibody (Cat# AAA1750456)
Full Name
Anti-IL1RA Picoband Antibody
Gene Names
Il1rn; IL-1ra
Reactivity
Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (MBS1750473).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD. )
MIP-3 Alpha/CCL20, Polyclonal Antibody (Cat# AAA1750473)
Full Name
Anti-MIP-3 Alpha/CCL20 Picoband Antibody
Gene Names
Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-mTOR (S2448) expression in HEK293 cell lysate (MBS1750174).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR, Monoclonal Antibody (Cat# AAA1750174)
Full Name
Anti-Phospho-mTOR (S2448) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of AFP expression in HepG2 cell lysate (MBS179349).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AFP )
AFP/Alpha 1 Fetoprotein, Monoclonal Antibody (Cat# AAA179349)
Full Name
Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody
Gene Names
AFP; AFPD; FETA; HPAFP
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SDF1 expression in Jurkat cell lysate (MBS179065).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL12 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCL12 )
SDF1, Monoclonal Antibody (Cat# AAA179065)
Full Name
Anti-SDF1 Rabbit Monoclonal Antibody
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12
Reactivity
Human
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of LOX-1/OLR1 using anti-LOX-1/OLR1 antibody (MBS1750479).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX-1/OLR1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LOX-1/OLR1 at approximately 38KD. The expected band size for LOX-1/OLR1 is at 31KD. )
LOX-1/OLR1, Polyclonal Antibody (Cat# AAA1750479)
Full Name
Anti-LOX-1/OLR1 Picoband Antibody
Gene Names
OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-mTOR (S2481) expression in (1)293 cell lysate treated with LP; (2)293 cell lysate (MBS1750175).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR, Monoclonal Antibody (Cat# AAA1750175)
Full Name
Anti-Phospho-mTOR (S2481) Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of KGF using anti-KGF antibody (MBS1750731).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates,Lane 2: rat RH35 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KGF antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KGF at approximately 22KD. The expected band size for KGF is at 22KD. )
KGF, Polyclonal Antibody (Cat# AAA1750731)
Full Name
Anti-KGF Picoband Antibody
Gene Names
Fgf7; Kgf
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of IP10 expression in IP10 recombinant protein lysate (MBS179236).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL10 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCL10 )
IP10, Monoclonal Antibody (Cat# AAA179236)
Full Name
Anti-IP10 Rabbit Monoclonal Antibody
Gene Names
CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of IL1RA using anti-IL1RA antibody (MBS1750455).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.Lane 1: recombinant mouse IL1RA protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1RA antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL1RA at approximately 17KD. The expected band size for IL1RA is at 17KD. )
IL1RA, Polyclonal Antibody (Cat# AAA1750455)
Full Name
Anti-IL1RA Picoband Antibody
Gene Names
Il1rn; IL-1ra; F630041P17Rik
Reactivity
Mouse
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of ERK1/2 Antibody expression in HepG2 whole cell lysates (MBS179108).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK3 )
ERK1/2, Monoclonal Antibody (Cat# AAA179108)
Full Name
Anti-ERK1/2 Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human gastric carcinoma, using Phospho-Creb (S133) Antibody(MBS1750236)CREB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CREB1 Antibody (MBS1750236)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Creb, Monoclonal Antibody (Cat# AAA1750236)
Full Name
Anti-Phospho-Creb (S133) Rabbit Monoclonal Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of mTOR expression in Jurkat cell lysate (MBS179041).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR/Tor, Monoclonal Antibody (Cat# AAA179041)
Full Name
Anti-mTOR/Tor Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (MBS1750516).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30KD. The expected band size for IGFBP1 is at 30KD. )
IGFBP1, Polyclonal Antibody (Cat# AAA1750516)
Full Name
Anti-IGFBP1 Picoband Antibody
Gene Names
IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
Reactivity
Human
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
ERG, Assay Kit (Cat# AAA3224720)
Full Name
ERG Western Blot kit
Gene Names
ERG; p55; erg-3
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
CLCN3, Assay Kit (Cat# AAA3224750)
Full Name
CLCN3 Western Blot kit
Gene Names
CLCN3; CLC3; ClC-3
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
CLIC1, Assay Kit (Cat# AAA3224748)
Full Name
CLIC1 Western Blot kit
Gene Names
CLIC1; G6; CL1C1; NCC27
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
Western Blot (WB)
(Western blot analysis of CREB expression in NIH/3T3 cell lysate (MBS179376).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CREB1 )
CREB, Monoclonal Antibody (Cat# AAA179376)
Full Name
Anti-CREB Rabbit Monoclonal Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
HIPK2, Assay Kit (Cat# AAA3224732)
Full Name
HIPK2 Western Blot kit
Gene Names
HIPK2; PRO0593
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
FST, Assay Kit (Cat# AAA3224792)
Full Name
FST Western Blot kit
Gene Names
FST; FS
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
RIPA, Lysis Buffer (Cat# AAA2557000)
Full Name
RIPA Lysis Buffer
Applications
WB
Pricing
$85/20 mL | $115/50 mL | $125/100 mL | $545/5x100 mL
RORA, Assay Kit (Cat# AAA3224800)
Full Name
RORA Western Blot kit
Gene Names
RORA; ROR1; ROR2; ROR3; RZRA; NR1F1; IDDECA; RZR-ALPHA
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
EED, Assay Kit (Cat# AAA3224766)
Full Name
EED Western Blot kit
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
RNF2, Assay Kit (Cat# AAA3224744)
Full Name
RNF2 Western Blot kit
Gene Names
Sce; CG5595; Ding; DmelCG5595; dring; dRing; dRING; Dring; DRING; dRing1; dRING1; Ring; RING; Ring/Sce; RING/Sce; RING1; Rnf2; sce; SCE; Sce/dRing
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
ENG, Assay Kit (Cat# AAA3224736)
Full Name
ENG Western Blot kit
Gene Names
en; 153867_at; Apa; CG9015; DmelCG9015; En; EN; en1; Eng; Engr; Engrailed; Engrailed/Invected; EnR; Es; spa2; spt; V
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
STAU1, Assay Kit (Cat# AAA3224773)
Full Name
STAU1 Western Blot kit
Gene Names
STAU1; STAU; PPP1R150
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
ASPN, Assay Kit (Cat# AAA3224794)
Full Name
ASPN Western Blot kit
Gene Names
ASPN; OS3; PLAP1; PLAP-1; SLRR1C
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
SSB, Assay Kit (Cat# AAA3224772)
Full Name
SSB Western Blot kit
Gene Names
SSB; La; LARP3; La/SSB
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
KCNN2, Assay Kit (Cat# AAA3224749)
Full Name
KCNN2 Western Blot kit
Gene Names
KCNN2; SK2; hSK2; SKCA2; KCa2.2; SKCa 2
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
PANX2, Assay Kit (Cat# AAA3224796)
Full Name
PANX2 Western Blot kit
Gene Names
PANX2; PX2; hPANX2
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
RHOBTB1, Assay Kit (Cat# AAA3224787)
Full Name
RHOBTB1 Western Blot kit
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
ATP7A, Assay Kit (Cat# AAA3224740)
Full Name
ATP7A Western Blot kit
Gene Names
ATP7A; MK; MNK; DSMAX; SMAX3
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
AHR, Assay Kit (Cat# AAA3224728)
Full Name
AHR Western Blot kit
Gene Names
AHR; bHLHe76
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
TGFBI, Assay Kit (Cat# AAA3224798)
Full Name
TGFBI Western Blot kit
Gene Names
TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
PAX7, Assay Kit (Cat# AAA3224730)
Full Name
PAX7 Western Blot kit
Gene Names
PAX7; HUP1; RMS2; PAX7B
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
ZBP1, Assay Kit (Cat# AAA3224760)
Full Name
ZBP1 Western Blot kit
Gene Names
IGF2BP1; IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
ZFP42, Assay Kit (Cat# AAA3224756)
Full Name
ZFP42 Western Blot kit
Gene Names
ZFP42; REX1; REX-1; ZNF754; zfp-42
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
TARDBP, Assay Kit (Cat# AAA3224767)
Full Name
TARDBP Western Blot kit
Gene Names
TARDBP; ALS10; TDP-43
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
SATB1, Assay Kit (Cat# AAA3224765)
Full Name
SATB1 Western Blot kit
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
HTR1A, Assay Kit (Cat# AAA3224722)
Full Name
HTR1A Western Blot kit
Gene Names
HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
KEAP1, Assay Kit (Cat# AAA3224747)
Full Name
KEAP1 Western Blot kit
Gene Names
KEAP1; INrf2; KLHL19
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
ILF2, Assay Kit (Cat# AAA3224754)
Full Name
ILF2 Western Blot kit
Gene Names
ILF2; NF45; PRO3063
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
TNRC4, Assay Kit (Cat# AAA3224724)
Full Name
TNRC4 Western Blot kit
Gene Names
CELF3; CAGH4; ERDA4; ETR-1; TNRC4; BRUNOL1
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
TRPM3, Assay Kit (Cat# AAA3224752)
Full Name
TRPM3 Western Blot kit
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
PANX1, Assay Kit (Cat# AAA3224797)
Full Name
PANX1 Western Blot kit
Gene Names
PANX1; PX1; MRS1; UNQ2529
Pricing
$665/10 Reactions | $2,840/5x10 Reactions
