RIPA Lysis Buffer
RIPA Lysis Buffer
Applications
Western Blot
Synonyms
RIPA; RIPA Lysis Buffer; RIPA lysis buffer
Specificity
The Kit for Rift Valley Fever Virus (RVFV) genomes is designed for the in vitro quantification of RVFV genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design. ynamics of genetic variation means that new sequence information may become available after the initial design. Periodically reviews the detection profiles of our kits and when required releases new versions.
Sequence Length
227
Applicable Applications for RIPA lysis buffer
Western Blot (WB)
Related Product Information for RIPA lysis buffer
Background/Introduction: Rift Valley fever is an arthropod-borne viral anthropozoonosis caused by a single stranded RNA phlebovirus called Rift Valley fever virus (RVFV). RVFV is transmitted by mosquitoes and causes severe hemorrhagic illness among humans, while in livestock it causes fever and high abortion rates. The genome consists of three negative sense, single stranded RNA segments designated L (large), M (medium) and S (small). The RVFV L segment (6.4 kb) encodes an RNA-dependent RNA polymerase, the M segment (3.9 kb) encodes two structural glycoproteins (G1 and G2) and two proteins of unknown function (NSm and 78-kDa protein), and the S segment (1.7 kb) encodes a nucleoprotein (N) in the anti-viral sense and a nonstructural protein (NSs) in the viral sense. Rift valley fever virus primarily spreads through transcutaneous transmission, aerosol transmission and insect bites. RVF is most commonly associated with mosquito-borne epidemics during years of unusually heavy rainfall. The mosquitoes, usually of the genus Aedus, breed in stagnant ponds that have been created by the heavy rainfall. As the mosquitoes grow in number the epizootic and epidemic cycles can occur. The mosquito eggs are naturally infected with the RVF virus, and the resulting mosquitoes transfer the virus to the livestock on which they feed. Once the livestock is infected, other species of mosquitoes can become infected from the animals and can spread the disease. During dry periods, the virus lies dormant in drought-resistant mosquito eggs over much of the African continent. The virus is also transmitted via contact with the blood, secretions, or excretions of infected animals. Because the virus affects livestock, contact with diseased animals can be via slaughtering or handling of infected animals, herding, touching contaminated meat from infected animals, and so on. RVFV forms plaques, usually targeting the liver and brain. After a mosquito bite, the virus moves from the skin to draining lymph nodes, where it replicates. Efferent lymphatics spread the virus throughout the body. As the liver is rapidly invaded, hepatocytes become involved. The virus may also cross the blood-brain barrier and infect neurons and glia. Patients suffering from RVF may show no symptoms at all, flu-like symptoms or sudden onset of mild fever with symptoms of liver and kidney disorder. More severe forms of the disease include the ocular, meningoencephalitis and haemorrhagic fever forms. Typically patients recover within 2 to 7 days after onset of illness. Approximately 1% of humans that become infected with RVFV die of the disease. Fatalities are significantly higher for infected livestock.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The RIPA (Catalog #AAA2557000) is a Lysis Buffer and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RIPA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the RIPA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIPA, Lysis Buffer" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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