AKAP5, CT (AKAP79, A-kinase Anchor Protein 5, AKAP-5, A-kinase Anchor Protein 79kD, AKAP 79, H21, cAMP-dependent Protein Kinase Regulatory Subunit II High Affinity-binding Protein) (Biotin)
Western Blot (WB) (Western Blotting Analysis: Representative lot data. HEK293 cell lysate with overexpressed N-terminal DYKDDDDK-AKAP79 was probed with Anti-AKAP 79 (1ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates AKAP 79 (~79kD). Uncharacterized bands appear at ~35kD and ~13kD in some lysates)
Testing Data (Recombinant IDH2 (R140Q) protein activity assay 20 uM NADPH and 1 uM a-Ketoglutaric acid were incubated with 500 nM IDH2 (R140Q) protein in a reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA at room temperature. Depletion of NADPH was monitored continuously at absorbance 340 nm for 60 min.)
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