SDS-Page
(Recombinant IDH2 (R140Q) protein 10% SDS-PAGE Coomassie staining MW: 52.6 kDa Purity: >95%)
IDH2 recombinant protein
Recombinant IDH2 (R140Q) protein
Gene Names
IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
Synonyms
IDH2; Recombinant IDH2 (R140Q) protein; Isocitrate Dehydrogenase (NADP(+)) 2; Mitochondrial; NADP(+)-Specific ICDH; IDH2 recombinant protein
Host
Baculovirus
Form/Format
Recombinant IDH2 (R140Q) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant IDH2 (R140Q) protein 10% SDS-PAGE Coomassie staining MW: 52.6 kDa Purity: >95%)
SDS-Page
(Recombinant IDH2 (R140Q) protein 10% SDS-PAGE Coomassie staining MW: 52.6 kDa Purity: >95%)
Testing Data
(Recombinant IDH2 (R140Q) protein activity assay 20 uM NADPH and 1 uM a-Ketoglutaric acid were incubated with 500 nM IDH2 (R140Q) protein in a reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA at room temperature. Depletion of NADPH was monitored continuously at absorbance 340 nm for 60 min.)
Testing Data
(Recombinant IDH2 (R140Q) protein activity assay 20 uM NADPH and 1 uM a-Ketoglutaric acid were incubated with 500 nM IDH2 (R140Q) protein in a reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA at room temperature. Depletion of NADPH was monitored continuously at absorbance 340 nm for 60 min.)
Related Product Information for IDH2 recombinant protein
Short Description: Recombinant IDH2 (R140Q) protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_002159.2) with a point mutation Arg140Gln and a C-terminal DYKDDDDK-Tag. The molecular weight of the protein is 52.6 kDa. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Mutations in IDH1 and IDH2 frequently occur in grade II-III gliomas, secondary glioblastomas and acute myeloid leukemias (AML). To date, mutations in at least four active site arginine residues IDH1 R100, IDH1 R132, IDH2 R140, and IDH2 R172 have been shown to result in the neomorphic production of R(-)-2-hydroxyglutarate (2HG), although these mutants lack the wild-type enzyme's ability to convert isocitrate to a-ketoglutarate (a-KG, 2OG).
Background: IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial, also known as MNADP-IDH, D2HGA2, IDHM, IDH, IDPM, IDC-M) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Mutations in IDH1 and IDH2 frequently occur in grade II-III gliomas, secondary glioblastomas and acute myeloid leukemias (AML). To date, mutations in at least four active site arginine residues IDH1 R100, IDH1 R132, IDH2 R140, and IDH2 R172 have been shown to result in the neomorphic production of R(-)-2-hydroxyglutarate (2HG), although these mutants lack the wild-type enzyme's ability to convert isocitrate to a-ketoglutarate (a-KG, 2OG).
Product Categories/Family for IDH2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
452
NCBI Official Full Name
isocitrate dehydrogenase
NCBI Official Synonym Full Names
isocitrate dehydrogenase 2 (NADP+), mitochondrial
NCBI Official Symbol
IDH2
NCBI Official Synonym Symbols
IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
NCBI Protein Information
isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase
UniProt Protein Name
Isocitrate dehydrogenase [NADP], mitochondrial
Protein Family
UniProt Gene Name
IDH2
UniProt Synonym Gene Names
IDH
UniProt Entry Name
IDHP_HUMAN
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Product Notes
The IDH2 idh2 (Catalog #AAA388314) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IDH2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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