The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Immunofluorescence (IF) (Immunofluorescence staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human CLU monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Human CLU on A549 cells. Cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Western Blot (WB) (Anti-CLU rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:52 kDa Observed band size:55 kDa)
Immunofluorescence (IF) (Immunofluorescence staining of CLU-His in HELA cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CLU-His polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.)
Western Blot (WB) (Western blot analysis of extracts from HepG2 serum starvation treated, using Phospho-PRKCA (Ser657) Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB) (Western blot analysis of PRKCA (Phospho-Ser657) using Forskolin treated HeLa whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)
Immunohistochemistry (IHC) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Testing Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
SDS-Page (SDS-Page)
Immunofluorescence (IF) (Immunofluorescence analysis of U-2 OS cells using SRSF7 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western blot detection of PKC alpha (phospho-Ser657) in mouse Brain,Ramos using PKC alpha (phospho-Ser657) Rabbit mAb(1:1000 diluted))
Immunohistochemistry (IHC) (Immunochemical staining of human GAPDH in human hepatitis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC) (Immunochemical staining of human GAPDH in human appendicitis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Western Blot (WB) (Western blot detection of GAPDH in Hela,CHO-K1,C6,Rat Brain,Jurkat cell lysates using GAPDH Rabbit mAb(1:500 diluted). Predicted band size:36kDa. Observed band size:36kDa.)
Immunohistochemistry (IHC) (Immunochemical staining of human PRKCA in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC) (Immunochemical staining of human PRKCA in human lung cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)
SDS-Page
Testing Data (Primary: Anti-GAPDH at 1/1000 dilution)
Immunohistochemistry (IHC)-Formalin (Formalin-fixed, paraffin-embedded human adrenal gland stained with Clusterin/APOJ Mouse Monoclonal Antibody (CLU/4730). )
Western Blot (WB) (Western Blot Analysis of human testis lysate using Clusterin/APOJ Mouse Monoclonal Antibody (CLU/4723). )