Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody

GAPDH monoclonal antibody

Cat. Num. AAA3006024

• Reactivity: Human, Mouse, Rat, Rabbit
• Applications: Western Blot
• Purity: affinity purified by Protein G
• Synonyms: GAPDH; Monoclonal Antibody; GAPDH monoclonal antibody; 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogenase liver;Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS; p38 component; OCT1 coactivator in S phase; 38-KD component; wu:fb33a10; anti-GAPDH antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Rabbit
• Clonality: Monoclonal
• Specificity: GAPDH Monoclonal Antibody detects endogenous levels of GAPDH protein.
• Purity/Purification: affinity purified by Protein G
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-GAPDH antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-5000
• Immunogen: Full length GAPDH protein purified from rabbit muscle
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Primary: Anti-GAPDH at 1/1000 dilution)

Related Product Information for anti-GAPDH antibody

Loading ControlGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

NCBI and Uniprot Product Information

• NCBI GI #: 126723533
• NCBI GeneID: 100009074
• NCBI Accession #: NP_001075722.1
• NCBI GenBank Nucleotide #: NM_001082253.1
• UniProt Accession #: P46406; Q4AC92
• Molecular Weight: 35,780 Da
• NCBI Official Full Name: glyceraldehyde-3-phosphate dehydrogenase
• NCBI Official Symbol: GAPDH
• NCBI Protein Information: glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH
• UniProt Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• UniProt Gene Name: GAPDH
• UniProt Synonym Gene Names: GAPD; GAPDH

Uniprot Description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation ().